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DOI: 10.1055/s-2002-26739
© Georg Thieme Verlag Stuttgart · New York
Production of Paclitaxel and Baccatin III in a 20-L Airlift Bioreactor by a Cell Suspension of Taxus wallichiana
Publikationsverlauf
May 28, 2001
August 5, 2001
Publikationsdatum:
02. Mai 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200204/lookinside/thumbnails/10.1055-s-2002-26739-1.jpg)
Abstract
A cell suspension culture of Taxus wallichiana (Himalayan Yew) was grown in shake flasks and a 20-L airlift bioreactor running for 28 days in a batch mode, and its capacity to accumulate paclitaxel and baccatin III was measured. When both culture types were in the highest productive state (from day 24 to day 28), there was a greater accumulation of paclitaxel and baccatin III in the bioreactor culture than in the shake flask culture (factor of 2.0 and 1.2, respectively). These increases in paclitaxel and baccatin III production cannot be related to the difference observed between the growth rates of both cultures, because when the bioreactor culture was at maximum productivity, its cell biomass, expressed in g L-1 of dry weight, was similar to that obtained in the shake flask culture. It seems that these improvements were mainly due to adequate aeration and mixing of the culture in the bioreactor. The maximum yield observed for paclitaxel (20.84 mg L-1 day 24) and baccatin III (25.67 mg L-1 day 28) represents a productivity of 0.90 mg L-1 d-1 and 0.93 mg L-1 d-1 respectively.
Key words
Taxus wallichiana cell cultures - airlift bioreactor - scale up - paclitaxel and baccatin III production
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Prof. M. Teresa Piñol
Laboratorio de Fisiologia Vegetal
Facultad de Farmacia. Universidad de Barcelona
Avda. Diagonal 643
08028 Barcelona
Spain
eMail: pinyol@farmacia.far.ub.es
Telefon: +43-93 4024492
Fax: +43-93 4029043