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DOI: 10.1055/s-2002-26750
© Georg Thieme Verlag Stuttgart · New York
Angiogenic Activity of β-Sitosterol in the Ischaemia/Reperfusion-Damaged Brain of Mongolian Gerbil
Publikationsverlauf
June 1, 2001
September 30, 2001
Publikationsdatum:
02. Mai 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200204/lookinside/thumbnails/10.1055-s-2002-26750-1.jpg)
Abstract
Aloe vera continues to be used for wound healing as a folk medicine. We previously reported that A. vera gel has angiogenic activity. In this study, we report upon the isolation of an angiogenic component β-sitosterol from A. vera and examination of its effect upon damaged blood vessels of the Mongolian gerbil. In a chick embryo chorioallantoic membrane assay, β-sitosterol was found to have an angiogenic effect. It enhanced new vessel formation in gerbil brains damaged by ischaemia/reperfusion, especially in the cingulated cortex and septal regions, in a dose-dependent fashion (up to 500 μg/kg, p < 0.05, n = 34 - 40). β-Sitosterol also enhanced the expressions of proteins related to angiogenesis, namely von Willebrand factors, vascular endothelial growth factor (VEGF), VEGF receptor Flk-1, and blood vessel matrix laminin (p < 0.05, n = 6). In addition, the intraperitoneal administration of β-sitosterol at 500 μg/kg/day for a period of 19 days significantly improved the motion recovery of ischaemia/reperfusion-damaged gerbils as assessed by rota-rod testing (p < 0.001, n = 10). Our results suggest that β-sitosterol has therapeutic angiogenic effects on damaged blood vessels.
Key words
β-Sitosterol - Aloe barbadensis - Asphodelaceae - Mongolian gerbil - ischaemia/reperfusion
References
- 1 Davis R H, Leitner M G, Russo J M, Byrne M E. Wound healing. Oral and topical activity of Aloe vera . J. Am. Podiatr. Med. Assoc.. 1989; 79 559-62
- 2 Dunphy J E. Modern biochemical concepts on the healing of wounds: wound healing. Baltimore; Williams and Wilkins 1974: pp. 22-31
- 3 Thompson W D, Harvey J A, Kazmi M A, Stout A J. Fibrinolysis and angiogenesis in wound healing. J. Pathol.. 1991; 165 311-8
- 4 Lee M J, Yoon S H, Lee S K, Chung M H, Park Y I, Sung C K, Choi J S, Kim K W. In vivo angiogenic activity of dichloromethane extracts of Aloe vera gel . Arch. Pharm. Res.. 1995; 18 332-5
- 5 Lee M J, Lee O H, Yoon S H, Lee S K, Chung M H, Park Y I, Sung C K, Choi J S, Kim K W. In vitro angiogenic activity of Aloe vera gel on calf pulmonary artery endothelial (CAPE) cells. Arch. Pharm. Res.. 1998; 21 260-5
- 6 Buonocore G, Perrone S, Bracci R. Free radicals and brain damage in the newborn. Biol. Neonate. 2001; 79 180-6
- 7 Marth H H, Bernaudin M, Petit E, Bauer C. Neuroprotection and angiogenesis: dual role of erythropoietin in brain ischemia. News Physiol. Sci.. 2000; 15 225-9
- 8 Krupinski J, Issa R, Bujny T, Slevin M, Kumar P, Kumar S. et al . A putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischaemic stroke in humans. Stroke. 1997; 28 564-73
- 9 Kaneshiro E S, Ellis J E, Jayasimhulu K, Beach D H. Evidence for the presence of ”Metabolic Sterols” in Pneumocytis: identification and initial characterization of Pneumocystis carinii sterols. J. Eukaryot. Microbiol.. 1994; 41 78-85
- 10 Lobb R R, Alderman E M, Fett J W. Induction of angiogenesis by bovine brain derived class 1 heparin-binding growth factor. Biochemistry. 1985; 24 4969-73
- 11 Glaser B M, D’amore P A, Michels R G, Patz A, Fenselau A. Demonstration of vasoproliferative activity from mammalian retina. J. Cell Biol.. 1980; 84 298-304
- 12 Dale N, Pearson T, Frenguelli B G. Direct measurement of adenosine release during hypoxia in the CA1 region of the rat hippocampal slice. J. Physiol.. 2000; 526 143-55
- 13 Choi Y, Fuchs E. TGF-beta and retinoic acid: regulators of growth and modifiers of differentiation in human epidermal cells. Cell Regul.. 1990; 1 791-809
- 14 Miranda S R, He X, Simonaro C M, Gatt S, Dagan A, Desnick R J, Schuchman E H. Infusion of recombinant human acid sphingomyelinase into Niemann-Pick disease mice leads to visceral, but not neurological, correction of the pathophysiology. FASEB J.. 2000; 14 1988-95
- 15 Jin K L, Mao X O, Greenberg D A. Vascular endothelial growth factor: Direct neuroprotective effect in in vitro ischaemia. Proc. Natl. Acad. Sci. USA. 2000; 97 10 242-7
- 16 Riekkinen P Jr, Schmidt B, Riekkinen M. Behavioral characterization of metrifonate-improved acquisition of spatial information in medial septum-lesioned rats. Eur. J. Pharmacol.. 1997; 26 11-9
- 17 Kita T, Nishijo H, Eifuku S, Terasawa K, Ono T. Place and contingency differential responses of monkey septal neurons during conditional place-object discrimination. J. Neurosci.. 1995; 15 1683-703
- 18 Levy D E, Van Uitert R L, Pike C L. Delayed postischaemic hypoperfusion: a potentially damaging consequence of stroke. Neurology. 1979; 29 1245-52
Myung-Hee Chung
Department of Pharmacology
Seoul National University
College of Medicine
Yongon-dong Chongnoku
Seoul 110-799
Korea
Telefon: +82-2-740-8294
Fax: +82-2-745-7996
eMail: mhchung@snu.ac.kr