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DOI: 10.1055/s-2002-26751
© Georg Thieme Verlag Stuttgart · New York
HPLC Analysis of Plasma 9-Methoxycanthin-6-one from Eurycoma longifolia and its Application in a Bioavailability/Pharmacokinetic Study
Publication History
May 18, 2001
September 30, 2001
Publication Date:
02 May 2002 (online)
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Abstract
A new and simple HPLC method using fluorescence detection was developed to determine 9-methoxycanthin-6-one, an active compound of Eurycoma longifolia Jack in rat and human plasma. The method entailed direct injection of plasma sample after deproteinization using acetonitrile. The mobile phase comprised acetonitrile and distilled water (55 : 45, v/v). Analysis was run at a flow rate of 1.0 ml/min with the detector operating at an excitation wavelength of 371 nm and emission wavelength of 504 nm. The method was specific and sensitive with a detection limit of 0.6 ng/ml and a quantification limit of approximately 1.6 ng/ml. The method was applied in a pilot pharmacokinetic/bioavailability study of the compound in rats. Less than 1 % of the compound was found to be absorbed orally.
Key words
Eurycoma longifolia - Simaroubaceae - 9-methoxycanthin-6-one - HPLC-analysis (fluorescence) - bioavailability
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Kah-Hay Yuen
School of Pharmaceutical Sciences
Universiti Sains Malaysia
11800 Penang
Malaysia
Email: khyuen@usm.my
Fax: +604-6596517