Prevention of Cellular ROS Damage by Isovitexin and Related Flavonoids

Chun-Mao Lin; Chien-Tsu Chen; Hsiao-Hui Lee; Jen-Kun Lin

doi:10.1055/s-2002-26753

Planta Medica, Table of Contents

Planta Med 2002; 68(4): 365-367
DOI: 10.1055/s-2002-26753

Letter

Prevention of Cellular ROS Damage by Isovitexin and Related Flavonoids

Chun-Mao Lin^1, ² , Chien-Tsu Chen² , Hsiao-Hui Lee¹ , Jen-Kun Lin¹

¹Institutes of Biochemistry and Molecular Biology, National Taiwan University, Taipei, Taiwan
²Department of Biochemistry, College of Medicine, Taipei Medical University, Taipei, Taiwan

Abstract

The antioxidant properties of isovitexin and related flavonoids were studied. Isovitexin inhibited xanthine oxidase with an IC₅₀ value of = 15.2 μM. The flavonoid analogues, apigenin, kaempferol, quercetin, myricetin, and genistein also inhibited xanthine oxidase with IC₅₀ values of 0.58, 2.18, 1.09, 9.90, and 4.83 μM, respectively. Isovitexin protected DNA from the Fenton reaction-induced breakage in a dose-dependent manner with an IC₅₀ value of 9.52 μM. Isovitexin also protected HL-60 cells from the ROS damage induced by the xanthine/xanthine oxidase reaction. Isovitexin exhibited the lowest cytotoxicity toward HL-60 cells (LD₅₀ >400 μM) compared to the other flavonoids examined. In addition, excess hydrogen peroxide induced by cadmium in A2780 ovarian cells was significantly suppressed by isovitexin. These results suggest that isovitexin in rice may protect cells from oxidative stress.

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Prof. Jen-Kun Lin

Institutes of Biochemistry and Molecular Biology,

College of Medicine, National Taiwan University

No. 1, Section 1

Jen-Ai Road

Taipei

Taiwan

Phone: +886-2-2356-2213

Fax: +886-2-2391-8944

Email: jklin@ha.mc.ntu.edu.tw