Apoptosis of Human Highly Metastatic Lung Cancer Cell Line 95-D Induced by Acutiaporberine, a Novel Bisalkaloid Derived from Thalictrum acutifolium

Qi Chen; Wenlie Peng; Suijian Qi; Anlong Xu

doi:10.1055/s-2002-32546

Planta Medica, Table of Contents

Planta Med 2002; 68(6): 550-553
DOI: 10.1055/s-2002-32546

Letter

Apoptosis of Human Highly Metastatic Lung Cancer Cell Line 95-D Induced by Acutiaporberine, a Novel Bisalkaloid Derived from Thalictrum acutifolium

Qi Chen¹ , Wenlie Peng¹ , Suijian Qi¹ , Anlong Xu¹

¹School of Life Sciences, Zhongshan (Sun Yatsen) University, Guangzhou, China

Abstract

The present study demonstrates for the first time that acutiaporberine, a bisalkaloid isolated from the tranditional Chinese medical herb Thalictrum acutifolium (Hand.-Mazz.) Boivin. (TAB), induces apoptosis of a cultured highly metastatic human lung cancer cell line 95-D. Immunohistochemistry assay (IHC) and Western-blot analysis show down-regulation of bcl-2 gene and up-regulation of bax gene and c-myc gene in the treated cells. These results suggest that acutiaporberine may be a natural potential apoptosis-inducing agent for highly metastatic lung cancer.

Abbreviations

TAB:Thalictrum acutifolium (Hand.-Mazz.) Boivin

FCM:flow cytometry

IHC:immunohistochemistry

TCM:traditional Chinese medicine

NCI:the National Cancer Institute of USA

DMSO:dimethyl sulphoxide

FBS:fetal bovine serum

PBS:phosphate buffered saline

PI:propidium iodide

EDTA:ethylenediaminetetraacetic acid disodium salt

UV:ultraviolet

DAB:diaminobenzidine

HRP:horseradish peroxidase streptavidin

Full Text

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Prof. Dr. Anlong Xu

Dept. of Biochemistry and

Center for Biopharmaceutical Research

School of Life Sciences

Zhongshan University

Guangzhou 510275

P R China

Phone: +86-20-8411 3655

Fax: +86-20-8403 8377

Email: ls36@zsu.edu.cn