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DOI: 10.1055/s-2002-32559
Studies on the Molecular Markers of Rhizomes of some Alpinia Species
Publikationsverlauf
September 29, 2001
January 12, 2002
Publikationsdatum:
01. Juli 2002 (online)
Abstract
The rhizomes of Alpinia jianganfeng are used as a traditional Chinese medicine, Jian Gan Feng, to cure rheumatism in Guangdong, China. The rhizomes of some other species of the genus Alpinia such as A. japonica, A. suishaensis and A. nanchuanensis are also used as Jian Gan Feng in Southwest China. However, the identification of the original plants of the crude drugs is difficult. The internal transcribed spacers and the 5.8S coding region of nuclear ribosomal DNA of the four species were sequenced and analyzed. The DNA markers have been determined and they can be used for the molecular identification of these medicinal plants.
References
- 1 Xie Z W. Quan Guo Zhong Cao Yao Hui Bian. Vol. 2 People’s Medical Publishing House 1996: 677-8
- 2 Jiangsu New Medical College . Zhong Yao Da Ci Dian. Vol. 1 Shanghai People’s Publishing House 1977: 169-70
- 3 Sichuan Institute of Chinese Materia Medica . Common Chinese Herbs in Sichuan Province. Sichuan People’s Publishing House 1971: 1345-6
- 4 Suh Y, Thien L B, Reeve H E, Zimmer E A. Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae. American Journal of Botany. 1993; 80 1042-55
- 5 Fu R Z, Wang J, Zhang Y B, Wang Z T, But P PH, Li N, Shaw P C. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism. Planta Medica. 1999; 65 48-50
- 6 Zhang Y B, Ngan F N, Wang Z T, Ng T B, But P PH, Shaw P C, Wang J. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities. Planta Medica. 1999; 65 157-60
- 7 Cai Z H, Li P, Dong T TX, Tsim K WK. Molecular diversity of 5S-rRNA spacer domain in Fritillaria species revealed by PCR analysis. Planta Medica. 1999; 65 360-4
- 8 Zhao Z L, Zhou K Y, Wang Z T, Qin M J, Dong H, Xu L S. Determination of nrDNA ITS sequences of Caodoukou (Alpinia hainanensis K. Schum.) and Yizhi (A. oxyphylla Miq.) Journal of Plant Resources and Environment. 2000; 9 38-40
- 9 Zhao Z L, Zhou K Y, Dong H, Xu L S. Characters of nrDNA ITS region sequences of fruits of Alpinia galanga and their adulterants. Planta Medica. 2001; 67 381-3
- 10 Baldwin B G, Sanderson M J, Porter J M, Wojciechowski M F, Campbell C S, Donoghue M J. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny. Annals of the Missouri Botanical Garden. 1995; 82 247-77
Professor Zhou Kaiya
Jiangsu Key Laboratory of Resources Biotechnology
Nanjing Normal University
Nanjing 210097
China
eMail: kyzhounj@jlonline.com
Fax: +86-25-3598328