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DOI: 10.1055/s-2002-32896
© Georg Thieme Verlag Stuttgart · New York
Inhibition of Telomerase Activity and bcl-2 Expression in Berbamine-Induced Apoptosis in HL-60 Cells
Publikationsverlauf
Received: October 22, 2001
Accepted: February 3, 2002
Publikationsdatum:
22. Juli 2002 (online)
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Abstract
The present study was aimed to investigate the effect of telomerase activity in berbamine-induced apoptosis and the regulation of B cell leukemia/lymphoma 2 (bcl-2) gene expression in human leukemia HL-60 cells. Apoptosis of HL-60 cells was induced by berbamine (10 μM) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase chain reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Berbamine induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the berbamine-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by berbamine. Inhibition of the telomerase activity of HL-60 cells was closely related to the berbamine-induced apoptosis. The present results indicate that inhibition of telomerase and reduced bcl-2 gene expression may play a role in the berbamine-induced apoptosis of HL-60 cells.
Key words
Berbamine - telomerase - apoptosis - bcl-2 gene - HL-60 cell - Menispermum dauricum
References
- 1 Kim N W, Piatyszek M A, Prowse K R, Harley C B, West M D, Ho P L. et al . Specific association of human telomerase activity with immortal cell and cancer. Science. 1994; 266 2011-5
- 2 Hiyama E, Gollahon L, Kataoka T, Kuroi K, Yokoyama T, Gazdar A F . et al . Telomerase activity in human breast tumor. Journal of National Cancer Institute. 1996; 88 116-22
- 3 Kondo Y, Kondo S, Tanaka Y, Haqqi T, Barna B P, Cowell J K. Inhibition of telomerase increase the susceptibility of human malignant glioblastoma cells to cisplatin-induced apoptosis. Oncogene . 1998; 16 2243-8
- 4 Mandal M, Kumar R. Bcl-2 modulates telomerase activity. J Biological Chemistry . 1997; 272 14 183-7
- 5 Fu W M, Begley J G, Killen M W, Mattson M P. Anti-apoptotic role of telomerase in pheochromocytoma cells. J Biological Chemistry. 1999; 274 7264-71
- 6 Zhang W. Mechanism of the protective effects of berbamine on ischemia-reperfusion injury in isolated rat heart. Zhonghua Xin Xue Guan Bing Za Zhi . 1993; 5 300-3
- 7 Alley M C, Scudiero D A, Monks A, Hursey M L, Czerwinski M J, Fine D L . et al . Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research. 1988; 48 589-601
- 8 Darzynkiewicz Z, Bino S BG, Gorczyca W. Features of apoptotic cells measured by flow cytometry. Cytometry . 1992; 13 795-9
- 9 Aiello A, Delia D, Borrello M G, Biassoni D, Giardini R, Fontanella E. et al . Flow cytometric detection of the mitochondrial Bcl-2 protein in normal and neoplastic human lymphoid cells. Cytometry . 1992; 13 502-9
- 10 Kim N W, Wu F. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleus Acids Research. 1997; 25 2595-7
- 11 Herbert B S, Pitts A E, Baker S I, Hamilton S E, Wright W E, Shay J W. et al . Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proceedings of National Academy of Sciences USA. 1999; 96 14 276-81
- 12 Bestilny L J, Brown C B, Miura Y, Robertson L D, Riabowol K T. Selective inhibition of telomerase activity during terminal differentiation of immortal cell lines. Cancer Research. 1996; 56 3796-802
- 13 Savoysky E, Yoshiida K, Ohtomo T, Yamaguchi Y, Akamatsu K I, Yamazaki T. et al . Down-regulation of telomerase activity id an early event in the differentiation of HL-60 cells. Biochemical and Biophysical Research Communications. 1996; 226 329-34
Prof. Guo-Qing Liu
Department of Pharmacology
China Pharmaceutical University
Nanjing 210009,
People’s Republic of China
Fax: +86-25-3271340
eMail: liugq@mailbox.cpu.edu.cn