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DOI: 10.1055/s-2002-32902
Effect of Acetazolamide on Hypericin Photocytotoxicity
Publikationsverlauf
Received: November 16, 2001
Accepted: February 9, 2002
Publikationsdatum:
22. Juli 2002 (online)
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Abstract
The photodynamic action of hypericin (HYP) in vitro was evaluated using human leukemic HL-60 and lung carcinoma A549 cell lines. After illumination HYP (1 × 10-5 M) reduced the proliferation and/or survival of HL-60 and A549 cells vs. controls to almost to 0 % and 29 %, respectively. A lower concentration of HYP (1 × 10-6 M) decreased the proliferation and/or survival only in HL-60 cells. Non-cytotoxic concentrations of the carbonic anhydrase inhibitor acetazolamide (ACTZ) (1 × 10-3-1 × 10-6 M) significantly potentiated these effects of HYP (1 × 10-6M) in HL-60, but not in the A549 cell line. The highest concentration of ACTZ (1 × 10-3 M) also induced an increase of the subdiploid G0/G1 population in HYP (1 × 10-6 M) treated HL-60 cells from 14 % to 24 %. The results indicate that the photogenerated pH drop may participate in the potentiation of the photodynamic action of HYP observed in leukemia cells.
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Dr. Peter Fedoročko
Department of Cellular and Molecular Biology
Faculty of Sciences, P.J. Šafárik University
Moyzesova 11
041 65 Košice
Slovakia
eMail: fedvox@kosice.upjs.sk
Fax: +421-55-6222124