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Horm Metab Res 2002; 34(7): 400-405
DOI: 10.1055/s-2002-33473
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Leptin Treatment during the Neonatal Period is Associated with Higher Food Intake and Adult Body Weight in Rats

C.  de Oliveira Cravo 1 , C.  V.  Teixeira1 , M.  C. F.  Passos 2 , S.  C. P.  Dutra 1 , E.  G.  de Moura 1 , C.  Ramos 1
  • 1Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Brazil
  • 2Department of Applied Nutrition, Nutrition Institute, State University of Rio de Janeiro, Brazil
Further Information

Publication History

Received: 24 September 2001

Accepted after revision: 4 February 2002

Publication Date:
21 August 2002 (online)

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Abstract

For this study, we have determined the effects of neonatal leptin treatment on the evolution of body weight. Experiment 1: pups were divided into two groups: LepF - injected with leptin (8 µg/100 g of body weight) for the first 10 days of lactation and control (C) - receiving saline. Experiment 2: pups were divided into two groups: LepL - injected with the same leptin concentration of experiment one for the last 10 days of lactation, and C, which received saline. Body weight and food intake were monitored until age 150 days, after which leptin concentrations were measured by ELISA. The LepF group had a significant increase in body weight (p < 0.05) from day 98 onward, in food intake (p < 0.05) from day 74 onward, and higher serum leptin concentration compared to the control (108 %, p < 0.05). The LepL group had a significant increase in body weight (p < 0.05) from day 113 onward, in food intake from day 121 onward (p < 0.001), and higher serum leptin concentration compared to controls (6.9 %, p < 0.05). These results suggest that both periods of lactation constituted a critical window for body weight and food intake programming, but the effects are more marked when the leptin is injected within the first ten days.

Key words

Neonatal - Leptin - Body Weight - Food Intake - Rats

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Dr. E. G. de Moura

Departamento de Ciências Fisiológicas, 5° andar · Instituto de Biologia · Universidade do Estado do Rio de Janeiro

Av. 28 de setembro 87 · Rio de Janeiro RJ, 20550-030 · Brazil ·

Phone: + 55 (21) 25 87 61 34

Fax: + 55 (21) 25 87 61 29

Email: egmoura@uerj.br