Antibodies to Bovine Beta-Casein in Diabetes and Other Autoimmune Diseases

L. Monetini; M. G. Cavallo; S. Manfrini; L. Stefanini; A. Picarelli; M. Di Tola; A. Petrone; M. Bianchi; M. La Presa; C. Di Giulio; M. G. Baroni; R. Thorpe; B. K. Walker; null IMDIAB Group; P. Pozzilli

doi:10.1055/s-2002-33595

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2002; 34(8): 455-459
DOI: 10.1055/s-2002-33595

Original Clinical

Antibodies to Bovine Beta-Casein in Diabetes and Other Autoimmune Diseases

L. Monetini ¹ , M. G. Cavallo ² , S. Manfrini ¹ , L. Stefanini ¹ , A. Picarelli ³ , M. Di Tola ³ , A. Petrone ⁴ , M. Bianchi ⁵ , M. La Presa ⁵ , C. Di Giulio ⁵ , M. G. Baroni ⁴ , R. Thorpe ⁶ , B. K. Walker ⁶ , IMDIAB Group ⁸ , P. Pozzilli ^1, ⁷

¹Unit of Diabetes and Endocrinology, University “Campus Bio-Medico”, Rome, Italy
²Dipartimento di Terapia Medica, University of Rome “La Sapienza”, Rome, Italy
³I Cattedra di Gastroenterologia, Dipartimento di Scienze Cliniche, University of Rome “La Sapienza”, Rome, Italy
⁴Endocrinologia, Dipartimento di Scienze Cliniche, University of Rome “La Sapienza”, Rome, Italy
⁵Dipartimento di Diagnostica, Ospedale Regina Apostulorum, Rome, Italy
⁶National Institute for Biological Standards and Control, South Mimms, UK
⁷Dept. of Diabetes and Metabolism, St. Bartholomew's Hospital, London, UK
⁸The IMDIAB Group: P. Pozzilli, F. Costanza, S. Manfrini, G. Coppolino, C. A. Mesturino, N. Visalli, M. Delli Castelli, A. Crinò, S. Corbi, S. Spera, C. Suraci, F. Costantino, A. Suppa, M. Cervoni, G. De Mattia, M. R. Cassone Faldetta, M. L. Manca Bitti, A. Cantagallo, M. C. Matteoli, P. Patera, E. Fioriti, D. Pitocco, G. Ghirlanda

Abstract

Cow's milk is thought to be an environmental trigger for autoimmune response in Type 1 diabetes. In the present study, our aim was to investigate the antibody response to bovine β-casein in different immune- and non-immune-mediated diseases and to establish whether such an antibody response is specific to Type 1 diabetes. We measured antibodies to bovine β-casein using an enzyme-linked immunosorbent assay in a total of 519 sera from subjects as follows: 71 patients with Type 1 diabetes, 33 patients with coeliac disease, 100 patients with latent autoimmune diabetes in adults (LADA), 50 patients with autoimmune thyroid disease (ATD), 50 patients with Type 2 diabetes, 24 patients with multiple sclerosis (MS), and 3 different groups of controls (n = 191). Significantly increased levels of antibodies to β-casein were found in patients with Type 1 diabetes, coeliac disease and in LADA compared to age-matched controls (p = 0.01, p = 0.02 and p = 0.01, respectively). No differences were observed in β-casein antibody titres between patients with other disease conditions (MS, and ATD) and age-matched controls. The highest antibody response to β-casein in Type 1 diabetic patients and in patients with coeliac disease could reflect the gut mucosal immune disorders common to Type 1 diabetes and coeliac disease. Furthermore, the elevated β-casein antibody levels found in LADA patients suggest that the antibody response to this protein may be relevant in autoimmune diabetes.

Key words

Cow's Milk - Type 1 Diabetes - Type 2 Diabetes - Autoimmune Diseases - β-Casein - Antibody

Full Text

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Prof. P. Pozzilli

University “Campus Bio-Medico”

Via Longoni 83 · 00155 Rome · Italy

Phone: + 39 (06) 22541556

Fax: + 39 (06 ) 22541336

Email: p.pozzilli@unicampus.it