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DOI: 10.1055/s-2002-33741
Effects of RRR-α-Tocopherol on Leukocyte Expression of HSP72 in Response to Exhaustive Treadmill Exercise
Publikationsverlauf
Accepted after revision: November 15, 2001
Publikationsdatum:
05. September 2002 (online)
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Abstract
Previous research revealed an increased expression of HSP72 in leukocytes after vigorous endurance exercise. We questioned whether more intensive but shorter exercise also induces leukocyte HSP72 synthesis. To delineate the role of reactive oxygen species (ROS) in exercise-related HSP72 induction, we additionally examined the effect of RRR-α-tocopherol (α-toc) on HSP72 expression using a double-blind placebo (P) controlled cross-over design. After supplementation with α-toc (500 I.U. daily) or P for 8 days, 9 male subjects performed a combined exhaustive treadmill protocol (total duration 29.4 ± 2.0 min). HSP72 was assessed on mRNA (RT-PCR) and protein levels (flow cytometry). HSP72 mRNA rose 3 h after exercise only in the P group, but individual differences (α-toc - P) did not reveal significant treatment effects. A moderate but significant rise of HSP72 protein occurred in granulocytes up to 48 h after exercise. Three hours post-exercise, granulocyte HSP72 protein was lower when subjects received α-toc, but this effect vanished 24 and 48 h post-exercise. Exhaustive treadmill exercise augments HSP72 mRNA in leukocytes and induced a moderate but prolonged response of granulocyte HSP72 protein. These exercise effects are lower when compared to earlier findings obtained after vigorous endurance exercise. ROS seem to be involved, but do not play the major role in the induction of granulocyte HSP72 synthesis after exhaustive exercise.
Key words
HSP72 - exercise - oxidative stress - antioxidants - RT-PCR - flow cytometry - immune function
References
- 1 Azzi A, Boscobonic D, Hensey C. The protein kinase family. Eur J Biochem. 1992; 208 547-557
- 2 Bachelet M, Mariethoz E, Banzet N, Souil E, Pinot F, Polla C Z, Durand P, Bouchaert P, Polla B S. Flow cytometry is a rapid and reliable method for evaluating heat shock protein 70 expression in human monocytes. Cell Stress Chaperones. 1998; 3 168-176
- 3 Baehner R L, Boxer L A, Allen J M, Davis J. Autooxidation as a basis for altered function by polymorphonuclear leucocytes. Blood. 1977; 50 327-335
- 4 Baehner R L, Boxer L A, Ingraham L M, Butterick C, Haak R A. The influence of vitamin E on human polymorphonuclear cell metabolism and function. Ann NY Acad Sci. 1982; 393 237-250
- 5 Biesalski H K, Greiff H, Brodda K, Hafner G, Baessler K H. Rapid determination of vitamin A (retinol) and vitamin E (alpha-tocopherol) in human serum by isocratic adsorptions HPLC. Int J Vit Nutr Res. 1986; 56 319-327
- 6 Cannon J G, Orencole S F, Fielding R A, Meydani M, Meydani S N, Fiatarone M A, Blumberg J B, Evans W J. Acute phase response in exercise: interaction of age and vitamin E on neutrophils and muscle enzyme release. Am J Physiol. 1990; 259 R1214-R1219
-
7 Dickhuth HH, Huonker M, Münzel T, Drexler H, Berg A, Keul J.
Individual anaerobic threshold for evaluation of competitive athletes and patients with left ventricular dysfunction. In: Bachl N, Graham TE, Löllgen H (eds) Advances in Ergometry. Berlin; Springer 1991: 173-179 - 8 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood plasma and red cells in dehydration. J Appl Physiol. 1974; 37 247-248
- 9 Dimitrov N V, Meyer C, Gilliland D, Ruppenthal M, Chenoweth W, Malone W. Plasma tocopherol concentrations in response to supplemental vitamin E. Am J Clin Nutr. 1991; 53 723-729
- 10 Essig D A, Nosek T M. Muscle fatigue and induction of stress protein genes: a dual function of reactive oxygen species?. Can J Appl Physiol. 1997; 22 409-428
- 11 Fehrenbach E, Niess A M, Schlotz E, Passek F, Dickhuth HH, Northoff H. Transcriptional and translational regulation of heat shock proteins in leukocytes of endurance runners. J Appl Physiol. 2000; 89 704-710
- 12 Fehrenbach E, Niess A M, Veith R, Dickhuth HH, Northoff H. Changes of HSP72-expression in leukocytes are associated with adaption to exercise under conditions of high environmental temperature. J Leukoc Biol. 2001; 69 747-754
- 13 Fincato G, Polentarutti N, Sica A, Mantovani A, Colotta F. Expression of a heat-inducible gene of the HSP70 family in human myelomonocytic cells: regulation by bacterial products and cytokines. Blood. 1991; 77 579-586
- 14 Gardner M J, Altman D G. Statistics with Confidence - Confidence Intervals and Statistical Guidelines. Belfast; Universities Press 1989
- 15 Gorman A M, Heavey B, Creagh E, Cotter T G, Samali A. Antioxidant-mediated inhibition of the heat shock response leads to apoptosis. FEBS Lett. 1999; 445 98-102
- 16 Guarnieri C, Melandri G, Caldarera I, Cervi V, Semprini F, Branzi A. Spontaneous superoxide generation by polymorphonuclear leukocytes isolated from patients with stable angina after physical exercise. Int J Cardiol. 1992; 37 301-307
-
17 Hartmann A, Niess A M.
DNA damage in exercise. In: Sen CK, Packer L, Hanninen O (eds) Handbook of Oxidants and Antioxidants in Exercise. Amsterdam; Elsevier 2000: 195-217 - 18 Islam K N, Devaraj S, Jialal I. Alpha-Tocopherol enrichment of monocytes decreases agonist-induced adhesion to human endothelial cells. Circulation. 1998; 98 2255-2261
- 19 Jacquier-Sarlin M R, Fuller K, Dinh-Xuan A T, Richard M J, Polla B S. Protective effects of hsp70 in inflammation. Experientia. 1994; 50 1031-1038
- 20 Kelly D A, Tiidus P M, Houston M E, Noble E G. Effect of vitamin E deprivation and exercise training on induction of HSP70. J Appl Physiol. 1996; 81 2379-2385
- 21 Liu Y, Mayr S, Opitz-Gress A, Zeller C, Lormes W, Baur S, Lehmann M, Steinacker J M. Human skeletal muscle HSP70 response to training in highly trained rowers. J Appl Physiol. 1999; 86 101-104
- 22 Locke M. The cellular stress responses to exercise - Role of stress proteins. Exerc Sports Sci Rev. 1997; 25 105 - 136
- 23 Locke M, Noble E G, Atkinson B G. Exercising mammals synthesize stress proteins. Am J Physiol. 1990; 258 C723-C729
- 24 Locke M, Noble E G, Tanguay R M, Feild M R, Ianuzzo S E, Ianuzzo C D. Activation of heat-shock transcription factor in rat heart after heat shock and exercise. Am J Physiol. 1995; 268 C1387-C1394
- 25 Marini M, Frabetti F, Musiani D, Franceschi C. Oxygen radicals induce stress proteins and tolerance to oxidative stress in human lymphocytes. Int J Radiat Biol. 1996; 70 337-350
- 26 McDuffee A T, Senisterra G, Huntley S, Lepock J R, Sekhar K R, Meredith M J, Borrelli M J, Morrow J D, Freeman M L. Proteins containing non-native disulfide bonds generated by oxidative stress can act as signals for the induction of the heat shock response. J Cell Physiol. 1997; 171 143-151
- 27 Meydani M. Vitamin E. Lancet. 1995; 345 170-175
- 28 Mizzen L A, Welch W J. Characterization of the thermotolerant cell. I. Effects on protein synthesis activity and the regulation of heat-shock protein 70 expression. J Cell Biol. 1988; 106 1105-1116
- 29 Moseley P L. Exercise, stress, and the immune conversation. Exerc Sports Sci Rev. 2000; 28 128-132
- 30 Niess A M, Dickhuth HH, Northoff H, Fehrenbach E. Free radicals and oxidative stress in exercise - immunological aspects. Exerc Immunol Rev. 1999; 5 22-56
- 31 Niess A M, Passek F, Lorenz I, Schneider E M, Dickhuth HH, Northoff H, Fehrenbach E. Expression of the antioxidant stress protein heme oxygenase-1 (HO-1) in human leukocytes - Acute and adaptional responses to endurance exercise. Free Rad Biol Med. 1999; 26 184-192
- 32 Noble E G, Moraska A, Mazzeo R S, Roth D A, Olsson M C, Moore R L, Fleshner M. Differential expression of stress proteins in rat myocardium after wheel or treadmill run training. J Appl Physiol. 1999; 86 1696-1701
- 33 Paroo Z, Tiidus P M, Noble E G. Estrogen attenuates HSP72 expression in acutely exercised male rodents. Eur J Appl Physiol. 1999; 80 180-184
- 34 Pinot F, el-Yaagoubi A, Christie P, Dinh-Xuan A T, Polla B S. Induction of stress proteins by tobacco smoke in human monocytes: modulation by antioxidants. Cell Stress Chaperones. 1997; 2 156-161
- 35 Polla B S, Perin M, Pizurki L. Regulation and functions of stress proteins in allergy and inflammation. Clin Exp Allergy. 1993; 23 548-556
- 36 Polla B S, Stubbe H, Kantengwa S, Maridonneau-Parini I, Jacquier-Sarlin M R. Differential induction of stress proteins and functional effects of heat shock in human phagocytes. Inflammation. 1995; 19 363-378
- 37 Roecker K, Schotte O, Niess A M, Horstmann T, Dickhuth H H. Predicting competition performance in long-distance running by means of a treadmill test. Med Sci Sports Exerc. 1998; 30 1552-1557
- 38 Salo D C, Donovan C M, Davies K J. HSP70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise. Free Radic Biol Med. 1991; 11 239-246
-
39 Traber M G.
Vitamin E. In: Sen CK, Packer L, Hanninen O (eds) Handbook of Oxidants and Antioxidants in Exercise. Amsterdam; Elsevier 2000: 359-371 - 40 Trautinger F, Kokesch C, Klosner G, Knobler R M, Kindas-Mugge I. Expression of the 72-kD heat shock protein is induced by ultraviolet A radiation in a human fibrosarcoma cell line. Exp Dermatol. 1999; 8 187-192
- 41 Ushakova T, Melkonyan H, Nikonova L, Mudrik N, Gogvadze V, Zhukova A, Gaziev A I, Bradbury R. The effect of dietary supplements on gene expression in mice tissues. Free Radic Biol Med. 1996; 20 279-284
- 42 Vigh L, Maresca B, Harwood J L. Does the membrane's physical state control the expression of heat shock and other genes?. Trends Biochem Sci. 1998; 23 369-374
- 43 Wei Q, Xu X, Cheng L, Legerski R J, Ali-Osman F. Simultaneous amplification of four DNA repair genes and ß-actin in human lymphocytes by multiplex reverse transcriptase-PCR. Cancer Res. 1995; 55 5025-5029
- 44 Zhao Y, Wein A J, Levin R M. Assessment of stress gene mRNAs (HSP-27, 60 and 70) in obstructed rabbit urinary bladder using a semi-quantitative RT-PCR method. Mol Cell Biochem. 1995; 148 1-7
A. M. Niess, M.D.
Medical Clinic & Polyclinic · Department of Sports Medicine · University of Tuebingen
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