Investigation on the Supercritical CO2 Extraction of the Volatile Constituents from Juniperus communis Obtained under Different Treatments of the ”Berries” (Cones)

Paschalina Chatzopoulou; Andre de Haan; Stavros T. Katsiotis

doi:10.1055/s-2002-34402

Planta Medica, Table of Contents

Planta Med 2002; 68(9): 827-831
DOI: 10.1055/s-2002-34402

Original Paper

Analysis
© Georg Thieme Verlag Stuttgart · New York

Investigation on the Supercritical CO₂ Extraction of the Volatile Constituents from Juniperus communis Obtained under Different Treatments of the ”Berries” (Cones)

Paschalina Chatzopoulou¹ , Andre de Haan² , Stavros T. Katsiotis³

¹National Agricultural Research Foundation, Agricultural Research Centre of Macedonia & Thrace, Department of Aromatic and Medicinal Plants, Thessaloniki, Greece
²Laboratory for Process Equipment, School of Chemical Engineering, Polytechnic of Delft, The Netherlands
³Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece

Abstract

The present investigation reports the experimental data a) from the recovery and the composition of the extract under super critical fluid extraction from Juniperus communis L. ”berries” (cones), and b) their comparison with those of the essential oil obtained by hydrodistillation. For the extraction of the juniper oil different values of temperature and pressure were applied; furthermore, the degree of comminution of the plant material was also considered - a) integral ”berries” and b) comminuted ”berries”. The quality of the oil recovered from the ”berries” by supercritical carbon dioxide extraction was found to be highly dependent on the applied conditions. The comminution affected greatly the oil recovery and consequently the final composition of the extracts. Significant differences were recorded between the supercritical CO₂ extract and the distilled oil, the latter being more enriched in monoterpenoid hydrocarbons.

Key words

Juniperus communis - Cupressaceae - volatiles - supercritical CO₂ extraction - hydrodistillation - GC/MS analysis

Full Text

References

1 Lamparsky D, Klimes I. Neue Ergebnisse der Wacholderbeeröl-Analyse im Hinblick auf terpenoide Inhaltsstoffe. Parfümerie und Kosmetic 1985 66 553-6, 558-60
2 Vernin G, Boniface C, Metzger J, Chiglione C, Hammoud A, Suon N, Fraisse D, Parkanyi C. GC-MS Specma bank analysis of Juniperus communis needles and berries. Phytochemistry. 1988; 27 1061-4
3 Gelsomini N, Vidrich V, Fusi P, Michelozzi M. Capillary gas chromatography of the terpenic fraction of Juniperus comminis L. black, green, berry and leaf extracts. Journal of High Resolution Chromatography & Chromatography Communications. 1988; 11 218-20
4 Tasic S R, Menkovic N R, Ristic M S, Kovacevic N N, Samardzic Z J. Comparative studies of juniper berry oil from different regions of central Balkan. Acta Horticulturae. 1993; 344 574-7
5 Chatzopoulou P S, Katsiotis T S. Study of the essential oil from Juniperus communis ”berries” (cones) growing wild in Greece. Planta Medica. 1993; 59 554-6
6 Koukos P K, Papadopoulou K I. Essential oil of Juniperus communis L. grown in northern Greece: variation of fruit oil yield and composition. Journal of Essential Oil Research. 1997; 9 35-9
7 Chatzopoulou P S, Katsiotis T S. Procedures influencing the yield and the quality of the essential oil from Juniperus communis L. berries. Pharmaceutica Acta Helvetiae. 1995; 70 247-53
8 Stahl E, Quirin K W, Glatz A, Gerard D, Rau G. Developments in the field of high-pressure extraction of natural products with dense gases. Ber Bunsenges Phys Chem. 1984; 88 900-7
9 Brogle H. CO₂ in solvent extraction. Chemistry and Industry 1982: 385-90
10 Della Porta G, Taddeo R, D’Urso E, Reverchon E. Isolation of clove bud and star anise essential oil by supercritical CO₂ extraction. Food Science and Technology. 1998; 31 454-60
11 Poiana M, Fresa R, Mincione B. Supercritical carbon dioxide extraction of bergamot peels. Extraction kinetics of oil and its components. Flavour and Fragrance Journal. 1999; 14, 6 358-66
12 Sorensen J M, Katsiotis S T. Parameters influencing the yield and composition of the essential oil from cretan Vitex Agnus-castus fruits. Planta Medica. 2000; 66 245-50
13 Langezaal C R, Chandra A, Katsiotis S T, Scheffer J C, de Haan A B. Analysis of supercritical carbon dioxide extracts from cones and leaves of a Humulus lupulus L. cultivar. Journal of the Science of Food and Agriculture. 1990; 53 455-63
14 Katsiotis S T. Study of different parameters influencing the composition of hydrodistilled sweet fennel oil. Flavour and Fragrance Journal. 1988; 4 221-4
15 Tuan D Q, Ilangantileke S G. Liquid CO₂ extraction of essential oil from star anise fruits (Illicium verum H.) Journal of Food Engineering. 1997; 31 47-57
16 Reverchon E, Della Porta G, Senatore F. Supercritical CO₂ extraction and fractionation of lavender essential oil and waxes. Journal of Agriculture and Food Chemistry. 1995; 43 1654-8
17 Moyler D A, Heath H B. Liquid carbon dioxide extraction of essential oils. In: Flavors and Fragrances: a world perspective Proc 10th Int Congr of Essential Oils, Fragrances and Flavors Washington DC; 1986: 41-63
18 Kerrola K, Kallio H. Volatile compounds and odor characteristics of carbon dioxide extracts of coriander (Coriander sativum L.) fruits. Journal of Agriculture and Food Chemistry. 1993; 41 785-90
19 Poiana M, Sicari V, Mincione B. A comparison between the chemical compositions of the oil, solvent extract and supercritical carbon dioxide extract of Citrus medica cv. Diamante. Journal of Essential Oil Research. 1998; 10 145-52

Prof. Dr. Ass. Katsiotis Th Stavros

Department of Pharmaceutical Technology

School of Pharmacy

Aristotle University of Thessaloniki

POBox 1589

54124 Thessaloniki

Greece

Email: stakat@pharm.auth.gr

Phone: +030-310-997624

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