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Planta Med 2002; 68(9): 856-858
DOI: 10.1055/s-2002-34413
Letter
© Georg Thieme Verlag Stuttgart · New York

An LC/DAD-UV/MS Method for the Rapid Detection of Aristolochic Acid in Plant Preparations

Jean-Robert Ioset1 , Guy Emmanuel Raoelison1, 2 , Kurt Hostettmann1
  • 1Institut de Pharmacognosie et Phytochimie, BEP, Université de Lausanne, Lausanne, Switzerland
  • 2Laboratoire de Pharmacodynamie, Faculté des Sciences, Université d’Antananarivo, Antananarivo, Madagascar
Further Information

Publication History

Received: December 27, 2001

Accepted: April 21, 2002

Publication Date:
30 September 2002 (online)

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Abstract

A new method using direct on-line coupling between HPLC and UV-DAD/MS was developed for the rapid detection of aristolochic acid I in plant preparations. The use of both UV-DAD and MS detectors enables this method to be very selective. A ng range detection limit was determined for both UV and MS detections. A quantitative determination of aristolochic acid I in plant preparations was achieved using UV and MS detection. A thin layer chromatography assay was also developed for the preliminary detection of aristolochic acid I in complex mixtures.

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Prof. Dr. K. Hostettmann

Institut de Pharmacognosie et Phytochimie

B.E.P.

1015 Lausanne

Switzerland

Email: Kurt. Hostettmann @ipp.unil.ch

Fax: +41-021/692 45 65