Adenosine Triphosphate Release and Purinergic Regulation of Cholangiocyte Transport

Andrew P. Feranchak; J. Gregory Fitz

doi:10.1055/s-2002-34503

Seminars in Liver Disease, Table of Contents

Semin Liver Dis 2002; 22(3): 251-263
DOI: 10.1055/s-2002-34503

Adenosine Triphosphate Release and Purinergic Regulation of Cholangiocyte Transport

Andrew P. Feranchak¹ , J. Gregory Fitz²

¹Department of Pediatrics, University of Colorado Health Sciences Center, Denver, Colorado
²Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado

Abstract

ABSTRACT

The discovery of purinergic receptors on almost every cell type studied to date suggests that purinergic signaling is a fundamental process regulating cell and organ level functions. Purinergic receptors have been found on all principal liver cell types, including liver parenchymal cells, or hepatocytes, and biliary epithelial cells, or cholangiocytes. Both hepatocytes and cholangiocytes are capable of the regulated release of adenosine triphosphate (ATP), and both cell types express a range of purinergic receptors to mediate cellular processes. The role of extracellular nucleotides in liver function is presently being elucidated. Extracellular ATP, in addition to autocrine regulation of liver cell volume, has recently been shown to play an important role in paracrine signaling to coordinate specific hepatocyte and cholangiocyte cellular responses. The findings that (1) cholangiocytes are capable of the regulated release of ATP into bile, (2) ATP is present in bile in concentrations capable of stimulating purinergic receptors, and (3) P2 receptor stimulation results in brisk Cl^- channel activation and fluid secretion suggest an important role of extracellular ATP in the regulation of bile formation. This article highlights important developments in our understanding of the role of purinergic signaling in cholangiocyte transport and bile formation.

KEYWORDS

Bile - biliary epithelium - purinergic signaling - nucleotides - Cl^- channel - CFTR - P2 receptor

Full Text

References

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