A Novel Sialyl Lewis^x Analogue Attenuates Ischemia Reperfusion Injury in Rabbit Lung

Abstract

Background: We investigated the effects of OJ-R9545, a novel Sialyl Lewis^x analogue, on lung ischemia-reperfusion (IR) injury using an in vivo rabbit model. Methods: The left hilum of the lung was clamped for 110 minutes; the lung was then reperfused for 90 minutes. Either OJ-R9545 (10 mg/kg) or vehicle solution was administered from 10 minutes before reperfusion to 60 minutes after reperfusion in the OJ-R (+) and OJ-R (-) group (n = 6 in each group), respectively. The sham group (n = 3) underwent an identical procedure without ischemia. Results: Arterial oxygen tensions in the OJ-R (+) group were superior to those in the OJ-R (-) group from 30 to 90 minutes after reperfusion (p < 0.05 and p < 0.01). Lung wet/dry weight ratio and myeloperoxidase activity after reperfusion in the OJ-R (+) group were both significantly lower than the corresponding figures in the OJ-R (-) group (p < 0.05). The intrapulmonary leukocytes were significantly reduced in the OJ-R (+) group compared with those in the OJ-R (-) group (p < 0.01). Conclusions: OJ-R9545 attenuates lung IR injury by preventing leukocyte infiltration into the lung.

Key words

Sialyl Lewis^x analogue - Leukocyte infiltration - Lung ischemia reperfusion injury

References

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