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Int J Sports Med 2002; 23(7): 484-488
DOI: 10.1055/s-2002-35067
Physiology & Biochemistry

© Georg Thieme Verlag Stuttgart · New York

Myosin Heavy Chain Composition of Single Muscle Fibers in Male Distance Runners

M.  P.  Harber1 , P.  M.  Gallagher1 , J.  Trautmann1 , S.  W.  Trappe1
  • 1Human Performance Laboratory, Ball State University, Muncie, Indiana, USA
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Publication History

Accepted after revision: March 15, 2002

Publication Date:
28 October 2002 (online)

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Abstract

The purpose of this study was to characterize the myosin heavy chain (MHC) composition of single muscle fibers from the gastrocnemius of male collegiate distance (DIST; n = 7), middle-distance (MID; n = 6), and recreational runners (REC; n = 6). Additionally, mATPase histochemistry was used to serve as a comparison to previous studies and the single fiber MHC technique. SDS-PAGE of single muscle fibers revealed a higher proportion of MHC I in DIST compared to MID and REC (74.9 ± 4.3 vs 54.4 ± 2.8 vs 56.2 ± 2.9 %, respectively; p < 0.05), less MHC IIa/IIx in DIST compared to MID and REC (0.0 ± 0.0 vs 6.0 ± 2.4 vs 15.9 ± 4.2 %, respectively; p < 0.05), and more total hybrids (I/IIa+IIa/IIx+I/IIa/IIx) in REC than both run groups, DIST and MID (23.0 ± 3.3 vs 6.2 ± 1.1 vs 13.2 ± 2.6 %, respectively; p < 0.05). ATPase histochemistry (pH 4.54) revealed a higher percentage of type I fibers in DIST compared to MID and REC (71.1 ± 3.1 vs 56.3 ± 2.5 vs 59.8 ± 2.3 %, respectively; p < 0.05), a higher percentage of type IIa in MID compared to DIST and REC (43.3 ± 2.7 vs 28.5 ± 3.1 vs. 30.2 ± 3.1 %, p < 0.05), and a higher distribution of type IIb in REC than both run groups (10.0 ± 2.7 vs 0.4 ± 0.2 vs 0.4 ± 0.2 %, p < 0.05). These results suggest that distance running leads to an increase in MHC I expression, training for mid-distance events leads to a prevalence of MHC IIa, and run training leads to a decrease in hybrid fibers.

Key words

Human skeletal muscle - endurance training - hybrid - myosin isoform

References

  • 1 Andersen J L, Klitgaard H, Bangsbo J, Saltin B. Myosin heavy chain isoforms in single fibres from m. vastus lateralis of soccer players: effects of strength-training.  Acta Physiol Scand. 1994;  150 21-26
    PubMedSearch in Google Scholar
  • 2 Andersen J L, Klitgaard H, Saltin B. Myosin heavy chain isoforms in single fibres from m. vastus lateralis of sprinters: influence of training.  Acta Physiol Scand. 1994;  151 135-142
    PubMedSearch in Google Scholar
  • 3 Andersen J L, Schiaffino S. Mismatch between myosin heavy chain mRNA and protein distribution in human skeletal muscle fibers.  Am J Physiol. 1997;  272 C1881-1889
    PubMedSearch in Google Scholar
  • 4 Bergstrom J. Muscle electrolytes in man.  Scand J Clin Lab Invest Suppl. 1962;  68 1-110
    PubMedSearch in Google Scholar
  • 5 Betto D D, Zerbato E, Betto R. Type 1, 2A, and 2B myosin heavy chain electrophoretic analysis of rat muscle fibers.  Biochem Biophys Res Commun. 1986;  138 981-987
    CrossrefPubMedSearch in Google Scholar
  • 6 Bottinelli R, Canepari M, Pellegrino M A, Reggiani C. Force-velocity properties of human skeletal muscle fibres: myosin heavy chain isoform and temperature dependence.  J Physiol. 1996;  495 573-586
    PubMedSearch in Google Scholar
  • 7 Brooke M H, Kaiser K K. Muscle fiber types: how many and what kind?.  Arch Neurol. 1970;  23 369-379
    CrossrefPubMedSearch in Google Scholar
  • 8 Chi M M, Hintz C S, Coyle E F, Martin W H 3rd, Ivy J L, Nemeth P M, Holloszy J O, Lowry O H. Effects of detraining on enzymes of energy metabolism in individual human muscle fibers.  Am J Physiol. 1983;  244 C276-287
    PubMedSearch in Google Scholar
  • 9 Costill D L, Daniels J, Evans W, Fink W, Krahenbuhl G, Saltin B. Skeletal muscle enzymes and fiber composition in male and female track athletes.  J Appl Physiol. 1976;  40 149-154
    PubMedSearch in Google Scholar
  • 10 Costill D L, Fink W J, Pollock M L. Muscle fiber composition and enzyme activities of elite distance runners.  Med Sci Sports. 1976;  8 96-100
    PubMedSearch in Google Scholar
  • 11 Giulian G G, Moss R L, Greaser M. Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels.  Anal Biochem. 1983;  129 277-287
    CrossrefPubMedSearch in Google Scholar
  • 12 Gollnick P D, Armstrong R B, Saubert C W, Piehl K, Saltin B. Enzyme activity and fiber composition in skeletal muscle of untrained and trained men.  J Appl Physiol. 1972;  33 312-319
    PubMedSearch in Google Scholar
  • 13 Howald H. Training-induced morphological and functional changes in skeletal muscle.  Int J Sports Med. 1982;  3 1-12
    Thieme ConnectPubMedSearch in Google Scholar
  • 14 Jackson A S, Pollock M L. Practical assessment of body composition.  Physician and Sports Medicine. 1985;  13 76-90
    PubMedSearch in Google Scholar
  • 15 Klitgaard H, Bergman O, Betto R, Salviati G, Schiaffino S, Clausen T, Saltin B. Co-existence of myosin heavy chain I and IIa isoforms in human skeletal muscle fibres with endurance training.  Pflugers Arch. 1990;  416 470-472
    PubMedSearch in Google Scholar
  • 16 Klitgaard H, Zhou M, Richter E A. Myosin heavy chain composition of single fibres from m. biceps brachii of male body builders.  Acta Physiol Scand. 1990;  140 175-180
    CrossrefPubMedSearch in Google Scholar
  • 17 Klitgaard H, Zhou M, Schiaffino S, Betto R, Salviati G, Saltin B. Ageing alters the myosin heavy chain composition of single fibres from human skeletal muscle.  Acta Physiol Scand. 1990;  140 55-62
    PubMedSearch in Google Scholar
  • 18 Laemmili U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.  Nature. 1970;  227 680-685
    PubMedSearch in Google Scholar
  • 19 Parcell A C, Sawyer R, Christopherson D, Poole C. Myosin heavy chain composition and myosin ATPase fiber type in elite female track athletes.  Med Sci Sports. 2001;  33 147
    PubMedSearch in Google Scholar
  • 20 Peuker K, Pette D. Quantitative analyses of myosin heavy-chain mRNA and protein isoforms in single fibers reveal a pronounced fiber heterogeneity in normal rabbit muscles.  Eur J Biochem. 1997;  247 30-36
    CrossrefPubMedSearch in Google Scholar
  • 21 Prince F P, Hikida R S, Hagerman F C. Human muscle fiber types in power lifters, distance runners and untrained subjects.  Pflugers Arch. 1976;  363 19-26
    CrossrefPubMedSearch in Google Scholar
  • 22 Saltin B, Henriksson J, Nygaard E, Andersen P, Jansson E. Fiber types and metabolic potentials of skeletal muscles in sedentary man and endurance runners.  Ann N Y Acad Sci. 1977;  301 3-29
    CrossrefPubMedSearch in Google Scholar
  • 23 Smerdu V, Karsch-Mizrachi I, Campione M, Leinwand L, Schiaffino S. Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle.  Am J Physiol. 1994;  267 C 1723-1728
    PubMedSearch in Google Scholar
  • 24 Srere P. Citrate Synthase. In: Colonick S, Kaplan N (ed) Methods in Enyzmology. New York; Academic 1969 13: p5
    Search in Google Scholar
  • 25 Staron R S, Gohlsch B, Pette D. Myosin polymorphism in single fibers of chronically stimulated rabbit fast-twitch muscle.  Pflugers Arch. 1987;  408 444-450
    PubMedSearch in Google Scholar
  • 26 Tesch P A, Karlsson J. Muscle fiber types and size in trained and untrained muscles of elite athletes.  J Appl Physiol. 1985;  59 1716-1720
    PubMedSearch in Google Scholar
  • 27 Trappe S, Costill D, Thomas R. Effect of swim taper on whole muscle and single muscle fiber contractile properties.  Med Sci Sports Exerc. 2001;  33 48-56
    PubMedSearch in Google Scholar
  • 28 Williamson D L, Godard M P, Porter D A, Costill D L, Trappe S W. Progressive resistance training reduces myosin heavy chain coexpression in single muscle fibers from older men.  J Appl Physiol. 2000;  88 627-633
    PubMedSearch in Google Scholar
  • 29 Williamson D L, Gallagher P M, Carroll C C, Raue U, Trappe S W. Reduction in hybrid single muscle fiber proportions with resistance training.  J AppI Physiol. 2001;  91 1955-1961
    PubMedSearch in Google Scholar

S. Trappe, Ph. D.

Human Performance Laboratory · Ball State University

Muncie, IN 47306 · USA ·

Phone: +1 (765) 285-1145

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Email: strappe@bsu.edu