Markers of Coagulation, Fibrinolysis and Angiogenesis After Strenuous Short-Term Exercise (Wingate-Test) in Male Subjects of Varying Fitness Levels

Abstract

It was the aim of the study to analyse the haemostatic system during a high standardized intensive short-term (30 s) exercise (anaerobic Wingate test). Blood samples were taken from 15 male subjects before (t₀), and within 2 (t₁), 9 (t₂) and 30 min (t₃) after the test. We found that the partial thromboplastin time was markedly shortened, whereas the prothrombin time increased slightly from t₀ to t₁ (p < 0.002) and remained elevated (t₃, p < 0.046). Factor VIII increased from t₀ to t₁ (p < 0.001) and remained elevated as well (t₃, p < 0.001). Fibrin monomers were approximately 15 times higher immediately post-exercise (t₁, p < 0.001) and continued to be elevated (t₃, p < 0.004). The tissue plasminogen activator increased by 4 times after exercise (t₁, p < 0.001) and remained elevated (t₃, p < 0.002). The d-dimers increased from t₀ to t₁ (p < 0.001) as well and remained elevated (t₃, p < 0.005). Thrombopoietin concentrations were unchanged, whereas the vascular endothelial growth factor increased immediately post-exercise (t₀ to t₁, p < 0.011 resp. at t₂ p < 0.019) and returned to the control level at t₃ (p < 0.878). In conclusion, it was found that prothrombotic markers and, even more pronounced, those of the fibrinolytic system were increased. The study provides evidence that due to intensive short-term exercise the balance of the haemostatic system is shifted to a higher equilibrium. Theoretically, the data show that in the case of a subject with risk factors such as impaired fibrinolysis, unfavourable conditions cannot be excluded.

Physical exercise - blood - coagulation - fibrinolysis - thrombopoiesis - thrombopoietin - vascular endothelial growth factor

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