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DOI: 10.1055/s-2002-35649
© Georg Thieme Verlag Stuttgart · New York
Pananotin, a Potent Antifungal Protein from Roots of the Traditional Chinese Medicinal Herb Panax notoginseng
Publikationsverlauf
Received: November 16, 2001
Accepted: July 13, 2002
Publikationsdatum:
26. November 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200211/lookinside/thumbnails/10.1055-s-2002-35649-1.jpg)
Abstract
The roots of the sanchi ginseng, Panax notoginseng, were extracted with an aqueous buffer. The extract was chromatographed on a CM-cellulose column to remove extraneous unadsorbed proteins. The adsorbed fraction was dialyzed and chromatographed on Affi-gel blue gel. The adsorbed fraction was again collected, dialyzed and applied on a column of Mono S. The second peak was dialyzed and chromatographed on an FPLC-gel filtration Superdex 75 column. An antifungal protein with an N-terminal sequence similar to those of chitinases was isolated from the first peak which had a molecular mass of 35 kDa. The sequence was distinctive in that the third and ninth highly conserved N-terminal residues (C and G) were replaced by H and M, respectively. The protein inhibited mycelial growth in Coprinus comatus, Physalospora piricola, Botrytis cinerea, and Fusarium oxysporum with an IC50 of 100 nM, 1 μM, 630 nM and 560 nM, respectively. It inhibited cell-free translation with an IC50 of 630 nM. Its antifungal and translation-inhibitory activities were more potent than those of previously reported antifungal proteins. It inhibited human immunodeficiency virus-1 reverse transcriptase by 35.8 % at 12.6 μM and 24.7 % at 1.26 μM.
Key words
Panax notoginseng - Araliaceae - antifungal protein - pananotin
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T. B. Ng
Department of Biochemistry
Faculty of Medicine
The Chinese University of Hong Kong
Shatin, Hong Kong
China
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Telefon: +852-2609-6875