Tubuloside B from Cistanche salsa Rescues the PC12 Neuronal Cells from 1-Methyl-4-phenylpyridinium Ion-Induced Apoptosis and Oxidative Stress

Abstract

The neuroprotective effects of tubuloside B, one of the phenylethanoids isolated from the Chinese herbal medicine Cistanche salsa, on 1-methyl-4-phenylpyridinium ion (MPP⁺)-induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. PC12 cells treated with MPP⁺ underwent apoptotic death as determined by MTT assay, flow cytometry and DNA agarose gel electrophoresis; intracellular accumulation of reactive oxygen species (ROS) was measured by DCFH-DA staining with laser scanning confocal microscopy (LSCM). Simultaneous treatment with tubuloside B markedly attenuated MPP⁺-induced cytotoxicity, DNA fragmentation, and intracellular accumulation of ROS. These results strongly indicate that tubuloside B prevents MPP⁺-induced apoptosis and oxidative stress. Tubuloside B may be applied as an antiparkinsonian agent.

Key words

Cistanche salsa - Orobanchaceae - tubuloside B - apoptosis - reactive oxygen species - oxidative stress - Parkinson's disease

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