Abstract
The neuroprotective effects of tubuloside B, one of the phenylethanoids isolated from the Chinese herbal medicine Cistanche salsa, on 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. PC12 cells treated with MPP+ underwent apoptotic death as determined by MTT assay, flow cytometry and DNA agarose gel electrophoresis; intracellular accumulation of reactive oxygen species (ROS) was measured by DCFH-DA staining with laser scanning confocal microscopy (LSCM). Simultaneous treatment with tubuloside B markedly attenuated MPP+-induced cytotoxicity, DNA fragmentation, and intracellular accumulation of ROS. These results strongly indicate that tubuloside B prevents MPP+-induced apoptosis and oxidative stress. Tubuloside B may be applied as an antiparkinsonian agent.
Key words
Cistanche salsa
- Orobanchaceae - tubuloside B - apoptosis - reactive oxygen species - oxidative stress - Parkinson's disease
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Prof. Changling Li,
Prof. Xiaoping Pu
Department of Molecular and Cellular Pharmacology
School of Pharmaceutical Sciences
Peking University
Beijing 100083
China
eMail: lichangling@bjmu.edu.cn
Fax: +86-10-62092648
