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DOI: 10.1055/s-2002-35667
© Georg Thieme Verlag Stuttgart · New York
Tubuloside B from Cistanche salsa Rescues the PC12 Neuronal Cells from 1-Methyl-4-phenylpyridinium Ion-Induced Apoptosis and Oxidative Stress
Publikationsverlauf
Received: March 18, 2002
Accepted: June 29, 2002
Publikationsdatum:
26. November 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200211/lookinside/thumbnails/10.1055-s-2002-35667-1.jpg)
Abstract
The neuroprotective effects of tubuloside B, one of the phenylethanoids isolated from the Chinese herbal medicine Cistanche salsa, on 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. PC12 cells treated with MPP+ underwent apoptotic death as determined by MTT assay, flow cytometry and DNA agarose gel electrophoresis; intracellular accumulation of reactive oxygen species (ROS) was measured by DCFH-DA staining with laser scanning confocal microscopy (LSCM). Simultaneous treatment with tubuloside B markedly attenuated MPP+-induced cytotoxicity, DNA fragmentation, and intracellular accumulation of ROS. These results strongly indicate that tubuloside B prevents MPP+-induced apoptosis and oxidative stress. Tubuloside B may be applied as an antiparkinsonian agent.
Key words
Cistanche salsa - Orobanchaceae - tubuloside B - apoptosis - reactive oxygen species - oxidative stress - Parkinson's disease
References
- 1 Coyle J T, Puttfarken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science. 1993; 262 689-94
- 2 Gotz M E, Kunig G, Riederer P, Youdium M BH. Oxidative stress: free radical production in neural degeneration. Pharmacol Ther. 1994; 63 37-122
- 3 Olanow C W. A radical hypothesis for neurodegeneration. Trends Neurosci. 1993; 16 439-44
- 4 Akaneya Y, Takahash M, Hatanaka H. Involvement of free radicals in MPP+ neurotoxicity against rat dopaminergic neurons in culture. Neurosci Lett. 1995; 193 53-6
- 5 Ambrosio S, Espino A, Cutillas B, Bartrons R. MPP+ toxicity in rat striatal slices: relationship between non-selective effects and free radical production. Neurochem Res. 1996; 21 73-8
- 6 Xiong Q, Kadota S, Tani T, Namba T. Antioxidative effects of phenylethanoids from Cistanche deserticola . Biol Pharm Bull. 1996; 19 1580-5
- 7 Xiong Q, Tezuka Y, Kaneko T, Li H, Tran L Q, Hase K, Namba T, Kadota S. Inhibition of nitric oxide by phenylethanoids in activated macrophages. Eur J Pharmacol. 2000; 400 137-44
- 8 Greene L A, Tischler A S. Establishment of a noradrenergic clonal line of adrenal pheochromocytoma cells which respon to nerve growth factor. Proc Nat Acad Sci. 1976; 73 2424-8
- 9 Yao Z, Drieu K, Szweda L I, Papadopoulos V. Free-radicals and lipid peroxidation do not mediate β-amyloid induced neuronal cell death. Brain Res. 1999; 847 203-10
- 10 Yamamoto T, Yuyama K, Nakamura K, Kato T, Yamamoto H. Kinetic characterization of the nitric oxide toxicity for PC12 cells: effect of half-life time of NO release. Eur J Pharmacol. 2000; 397 25-33
- 11 Nicoletti I, Migliorati G, Pagliacci M C, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991; 139 271-9
- 12 Moore K J, Matlashewski G. Intracellular infection by Leishmania donovani inhibits macrophage apoptosis. J Immunol. 1994; 152 2930-7
- 13 Mark R J, Keller J N, Kruman I, Mattson M P. Basic FGF attenuates amyloid β-peptide-induced oxidative stress, mitochondrial dysfunction, and impairment of Na+-K+-ATPase activity in hippocampal neurons. Brain Research. 1997; 756 205-14
- 14 Merad-Boudia M, Nicole A, Santiard-Baron D, Saille C, Cebal-los-Picot I. Mitochondrial impairment as an early event in the process of apoptosis induced by glutathione depletion in neuronal cells: relevance to Parkinson's disease. Biochem Pharmacol. 1998; 56 645-55
- 15 Langston J W, Ballard P, Tetrud J W, Irwin I. Chronic Parkinsonism in humans due to a product of meperidine-analog synthesis. Science. 1983; 219 979-80
- 16 Mizuno Y, Sone N, Suzuki K, Saitoh T. Studies on the toxicity of 1-methyl-4-phenylpyridinium ion (MPP+) against mitochondria of mouse brain. J Neurol Sci. 1988; 86 97-110
Prof. Changling Li,
Prof. Xiaoping Pu
Department of Molecular and Cellular Pharmacology
School of Pharmaceutical Sciences
Peking University
Beijing 100083
China
eMail: lichangling@bjmu.edu.cn
Fax: +86-10-62092648