Antimicrobial Activity of the Essential Oil and Some Isolated Sulfur-Rich Compounds from Scorodophloeus zenkeri

Abstract

The essential oil and several pure sulfur compounds isolated from Scorodophloeus zenkeri were tested for their antibacterial and antifungal activity using a paper disc method, the poisoned food technique, a microatmosphere method and the measurement of cellular ATP content. The essential oil completely inhibited the growth of all fungi tested including yeasts, with the exception of Aspergillus flavus, and was active against the Gram-positive bacteria studied, but not the Gram-negative organisms. 2,4,5,7-Tetrathiaoctane, 2,4,5,6,8-pentathianonane, 2,3,4,6,8-pentathianonane, 2,3,5,6,8,10-hexathiaundecane, 2,3,5-trithiahexane 5-oxide, 2,4,5,7-tetrathiaoctane 2-oxide, 2,3,5,7-tetrathiaoctane 3,3-dioxide and 2,3,5-trithiahexane 3,3-dioxide differed in their effects on the strains studied with respect to both growth and synthesis of cellular ATP. 2,3,5-Trithiahexane, 2,3,4,6-tetrathiaheptane, methyl methanethiosulfonate and bis-methyl-sulfonylmethane exhibited no antimicrobial activity.

Scorodophloeus zenkeri - Caesalpiniaceae - essential oil - sulfur compounds - antimicrobial activity

References

• 1 Chen I S, Chen H F, Cheng M J, Chang Y L, Teng C M, Tsutomu I. et al . Quinoline alkaloids and other constituents of Melicope semecarpifolia with antiplatelet aggregation activity.  Journal of Natural Products. 2001;  64 1143-7
• 2 Vairappan C S, Suzuki M, Abe T, Masuda M. Halogenated metabolites with antibacterial activity from the Okinawan Laurencia species.  Phytochemistry. 2001;  58 517-23
• 3 Resch M, Heilmann J, Steigel A, Bauer R. Further phenols and polyacetylenes from the rhizomes of Atractylodes lancea and their anti-inflammatory activity.  Planta Medica. 2001;  67 437-42
• 4 Aubréville A, Leroy J F. Flore du Cameroun. 9 Légumineuses-Césalpinioidées. Muséum National d’Histoire Naturelle, Laboratoire de Phanérogamie 1970: P. 83-6
• 5 Amvam Zollo P H, Dupont Youngo M J, Fekam B F, Menut C, Lamaty G, Bessière J M. Etude comparée de la composition chimique des huiles essentielles extraites à partir d’un ”arbre à ail”: Scorodophloeus zenkeri Harms (Caesalpiniacee) et de l’ail: Allium sativum Linn. (liliacee) du Cameroun. Actes des 14èmes Journées Internationales Huiles Essentielles, Digne-les-Bains 1995: 614-7
• 6 Hegnauer R, Hegnauer M. Chemotaxonomie der Pflanzen XIb-1 Leguminosae Teil 2. Birkhäuser Verlag Basel, Boston, Berlin; 1996: P. 152
• 7 Kouokam J C, Zapp J, Becker H. Isolation of new alkylthiosulfides from the essential oil and extracts from the bark of Scorodophloeus zenkeri Harms.  Zeitschrift für Naturforschung. 2001;  56c 1003-7
• 8 Kouokam J C, Zapp J, Becker H. Oxygen-containing sulfur-rich compounds from the bark of the tropical garlic tree Scorodophloeus zenkeri Harms.  Phytochemistry. 2002;  60 403-7
• 9 Dannhardt G, Grobe A, Gußmann S. 5-(3-Aminopropyl)-isoxazole: Synthese und antibakterielle Aktivität einiger Derivate.  Archiv der Pharmazie. 1988;  321 163-6
• 10 Ioset J R, Marston A, Gupta M P, Hostettmann K. Antifungal and larvicidal meroterpenoid naphthoquinones and a naphthoxirene from the roots of Cordia linnaei .  Phytochemistry. 1998;  47 729-34
• 11 Grover R K, Moore J D. Toximetric studies of fungicides against the brown rot organisms, Sclerotinia fructicola and S. laxa .  Phytopathology. 1962;  52 876-80
• 12 Mishra A K, Dubey N K. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities.  Applied and Environmental Microbiology. 1994;  60 (4) 1101-5
• 13 Jahns T. Ammonium/urea-dependent generation of a proton electrochemical potential and synthesis of ATP in Bacillus pasteurii .  Journal of Bacteriology. 1996;  178 (2) 403-9
• 14 Benjilali B, Tantaoui-Elakari A, Ayadi A, Ihlal M. Method to study antimicrobial effects of essential oils: application to the antifungal activity of six Moroccan essences.  Journal of Food Protection. 1984;  47 748-52
• 15 Naigre R, Kalck P, Roques C, Roux I, Michel G. Comparison of antimicrobial properties of monoterpenes and their carbonylated products.  Planta Medica. 1996;  62 275-7
• 16 Lim H, Kubota K, Kobayashi A, Seki T, Ariga T. Inhibitory effect of sulfur containing compounds in Scorodocarpus borneensis Becc. on the aggregation of rabbit platelets.  Bioscience, Biotechnology and Biochemistry. 1999;  63 (2) 298-301
• 17 Kubota K, Ohhira S, Kobayashi A. Identification and antimicrobial activity of the volatile flavor constituents from Scorodocarpus borneensis Becc.  Journal of Bioscience, Biotechnology and Biochemistry. 1994;  52 (4) 644-6