Planta Med 2003; 69(1): 33-37
DOI: 10.1055/s-2003-37027
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Protective Effects of Honokiol and Magnolol on Tertiary Butyl Hydroperoxide- or D-Galactosamine-Induced Toxicity in Rat Primary Hepatocytes

Eun-Jeon Park1 , Yu-Zhe Zhao1 , MinKyun Na2 , KiHwan Bae2 , Young Ho Kim2 , Byung-Hoon Lee1 , Dong Hwan Sohn1
  • 1Department of Pharmacy, Medicinal Resources Research Center, Wonkwang University, Iksan, Cheonbuk, Korea
  • 2College of Pharmacy, Chungnam National University, Daejeon, Korea
Further Information

Publication History

Received: April 15, 2002

Accepted: August 13, 2002

Publication Date:
04 February 2003 (online)

Abstract

The aim of this study was to investigate the protective effect of honokiol and magnolol on hepatocyte injury induced by either tertiary butyl hydroperoxide (tBH)- or D-galactosamine (GalN). The cellular leakage of LDH and AST, and cell death by treatment with 1.5 mM tBH for 1 h, were significantly inhibited by treatment with honokiol (40 and 20 μM) or magnolol (40 μM). Treatment with honokiol or magnolol significantly inhibited lipid peroxidation in both cells and media, the generation of intracellular reactive oxygen species (ROIs), and intracellular glutathione (GSH) depletion induced by tBH. The cellular leakage of LDH and AST, and cell death, by 24-hour treatment with 30 mM GalN were significantly inhibited by treatment with honokiol (20, 5 and 1 μM) or magnolol (20, 5 and 1 μM). Treatment with honokiol (20, 5 and 1 μM) or magnolol (20 and 5 μM) significantly inhibited the intracellular GSH depletion induced by GalN. The hepatoprotective effects of honokiol and magnolol on oxidative stress induced by tBH were probably the result of their antioxidant activity. Honokiol and magnolol also had a protective effect against GalN-induced hepatotoxicity, which was used as an alternate model to oxidative stress, acting by inhibiting intracellular GSH depletion.

Abbreviations

tBH:tertiary butyl hydroperoxide

GalN:D-galactosamine

AST:aspartate transaminase

LDH:lactate dehydrogenase

MTT:3-(4,5-dimethylthiazol-1-yl)-2,5-diphenyltetrazolium bromide

HBSS:Ca++-free Hanks' balanced salt

DMSO:dimethyl sulfoxide (DMSO)

TBARS:thiobarbituric acid reactive substance

GSH:glutathione (reduced form)

DCF:2′,7′-dichlorofluorescein

ROIs:reactive oxygen species

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Prof. Dong Hwan Sohn

Department of Pharmacy

Wonkwang University

Iksan, Cheonbuk 570-749

Republic of Korea

Email: dhsohn@wonkwang.ac.kr

Fax: +82 63 854 6038