Planta Med 2003; 69(1): 65-66
DOI: 10.1055/s-2003-37029
Letter
© Georg Thieme Verlag Stuttgart · New York

Acteoside from Cistanche salsa Inhibits Apoptosis by 1-Methyl-4-phenylpyridinium Ion in Cerebellar Granule Neurons

Xiaoping Pu1 , Zhihong Song2 , Yanyun Li1 , Pengfei Tu2 , Huinong Li1
  • 1Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science, Peking University, Beijing, P. R. China
  • 2Department of Natural Medicines, School of Pharmaceutical Science, Peking University, Beijing, P. R. China
Further Information

Publication History

Received: April 30, 2002

Accepted: August 13, 2002

Publication Date:
04 February 2003 (online)

Abstract

In this study we assessed the effect of acteoside that significantly improved cell viability and inhibited lactate dehydrogenase (LDH) release. Furthermore acteoside prevented a neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis in CGNs. Accordingly, our flow cytometric analysis of CGNs after acteoside treatment revealed a decrease in the number of the MPP+-induced apoptotic cells (P < 0.001). Western blot analysis demonstrated that acteoside inhibits the active caspase-3 fragment (17 kDa) (P < 0.001) and the proteolytic poly (ADP-ribose) polymerase (PARP) fragment (85 kDa) expression (P < 0.001) following MPP+ treatment in CGNs. We conclude that acteoside prevents the MPP+-induced apoptosis and inhibits the apoptosis-related pathway.

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Xiao-Ping Pu, Ph. D.

Department of Molecular and Cellular Pharmacology

School of Pharmaceutical Science

Peking University

Beijing 100083

P. R. China

Email: puxiaopingbj@163.com

Fax: +86-010-62092648,

Phone: +86-010-62092648