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DOI: 10.1055/s-2003-37705
Pulsatilloside A and Anemoside A3 Protect PC12 Cells from Apoptosis Induced by Sodium Cyanide and Glucose Deprivation
Publikationsverlauf
Received: May 21, 2002
Accepted: September 22, 2002
Publikationsdatum:
07. März 2003 (online)
![](https://www.thieme-connect.de/media/plantamedica/200302/lookinside/thumbnails/10.1055-s-2003-37705-1.jpg)
Abstract
Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A3, at dosages ranging from 0.1, 1 and 10 μg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.
Abbreviations
β-AP:β-amyloid protein
FASC:flow cytometry
LDH:lactate dehydrogenase
NGF:nerve growth factor
TEM:transmission electron microscope
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W. L. Wendy Hsiao
Department of Biology
The Hong Kong University of Science and Technology
Kowloon, Hong Kong
eMail: bowhsiao@ust.hk
Fax: 852-23581559