Pulsatilloside A and Anemoside A₃ Protect PC12 Cells from Apoptosis Induced by Sodium Cyanide and Glucose Deprivation

Abstract

Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A₃ on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A₃, at dosages ranging from 0.1, 1 and 10 μg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.

Abbreviations

β-AP:β-amyloid protein

FASC:flow cytometry

LDH:lactate dehydrogenase

NGF:nerve growth factor

TEM:transmission electron microscope

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