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Planta Med 2003; 69(2): 113-117
DOI: 10.1055/s-2003-37712
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Constituents of Sage (Salvia officinalis) with in vitro Affinity to Human Brain Benzodiazepine Receptor

Dominique Kavvadias1 , Vanessa Monschein1 , Philipp Sand2 , Peter Riederer2 , Peter Schreier1
  • 1Food Chemistry, University of Würzburg, Würzburg, Germany
  • 2Clinical Neurochemistry, University Hospital, Würzburg, Germany
Prof. Dr. Dr. h.c. Waldemar Adam’s 65th birthday
Further Information

Publication History

Received: June 24, 2002

Accepted: October 3, 2002

Publication Date:
07 March 2003 (online)

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Abstract

Benzodiazepine receptor binding assay-guided fractionation of the methanol extract from sage leaves (Salvia officinalis L.) revealed three flavones and two abietane diterpenes functioning as benzodiazepine receptor-active components. Structural elucidation of the isolated pure compounds was performed by UV, EI-MS, ESIpos-MS/MS, as well as 1H- and 13C-NMR techniques. The flavones apigenin, hispidulin and cirsimaritin competitively inhibited 3 H-flumazenil binding to the benzodiazepine receptor with IC50 values of 30, 1.3 and 350 μM, respectively. In addition, the affinities of the newly discovered diterpene receptor ligands, i. e., 7-methoxyrosmanol and galdosol, were characterized. 7-Methoxyrosmanol exhibited an IC50 value of 7.2 μM and galdosol showed the strongest binding activity to the benzodiazepine receptor with an IC50 value of 0.8 μM.

Key words

Salvia officinalis L. - Lamiaceae - hispidulin - apigenin - cirsimaritin - 7-methoxyrosmanol - galdosol - benzodiazepine receptor

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Prof. Dr. P. Schreier

Institut für Pharmazie und Lebensmittelchemie

Universität Würzburg

Am Hubland

97074 Würzburg

Germany

Email: schreier@pzlc.uni-wuerzburg.de

Fax: +49-931-888-5484