Planta Med 2003; 69(2): 155-157
DOI: 10.1055/s-2003-37716
Letter
© Georg Thieme Verlag Stuttgart · New York

Coumarins and Carbazoles from Clausena excavata Exhibited Antimycobacterial and Antifungal Activities

Arunrat Sunthitikawinsakul1 , Ngampong Kongkathip1 , Boonsong Kongkathip1 , Sida Phonnakhu1 , John W. Daly2 , Thomas F. Spande2 , Yuth Nimit3 , Sasarose Rochanaruangrai4
  • 1Natural Product and Organic Synthesis Research Unit (NPOS), Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
  • 2Laboratory of Bioorganic Chemistry, NIDDK, NIH, Bethesda, Maryland, USA
  • 3Office of National Advisory Council, NIH, Bethesda, Maryland, USA
  • 4National Center for Genetic Engineering and Biotechnology, NSTDA, 73/1, Bangkok, Thailand
Further Information

Publication History

Received: June 4, 2002

Accepted: October 27, 2002

Publication Date:
07 March 2003 (online)

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Abstract

Four known coumarins, dentatin (1), nor-dentatin (2), clausenidin (3) and xanthoxyletin (5), and six known carbazole derivatives, 3-formylcarbazole (6), mukonal (7), 3-methoxycarbonylcarbazole (8), murrayanine (9), 2-hydroxy-3-formyl-7-methoxycarbazole (10) and clauszoline J (11) were isolated from Clausena excavata. Compounds 1 and 6 were first isolated from the crude chloroform extract of the rhizomes. Compounds 1, 2, 3, 6, 7, 8, 10 and 11 showed antimycobacterial activity at a minimum inhibitory concentration (MIC) of 50, 100, 200, 100, 200, 50, 100 and 100 μg/mL, respectively. O-Methylated clausenidin (4), prepared from 3, exhibited antimycobacterial activity at MIC 50 μg/mL. Compounds 6, 7, 8 and 10 showed antifungal activity with IC50 values of 13.6, 29.3, 9.5 and 2.8 μg/mL, respectively. All compounds demonstrated no cytotoxicity against KB and BC-1 cell lines.