Abstract
In order to find new acetylcholinesterase inhibitors from natural sources which have therapeutic potential in the treatment of Alzheimer's disease, the chloroform : methanol (1 : 1) extracts of five Lycopodium species (L. clavatum L., L. selago L., L. annotinum L., L. alpinum L., and L. complanatum ssp. chamaecyparissus A. (Br.) Döll.), growing in Turkey (Lycopodiaceae), were screened in vitro by the Ellman spectrophotometric method at 1 mg/ml concentration. Among these extracts, only the extract of L. clavatum was found to be active (49.85 %). Bioassay-guided fractionation of the chloroform extract of L. clavatum led to the isolation of α-onocerin (IC50 = 5.2 μM), a well-known triterpenoid, as the responsible compound for the activity.
Abbreviations
AChE:Acetylcholinesterase
AD:Alzheimer's Disease
DTNB:5,5′-dithio-bis(2-nitrobenzoic acid)
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Dr. Ilkay Orhan
Department of Pharmacognosy
Faculty of Pharmacy
Gazi University
06330 Ankara-Turkey
eMail: iorhan@gazi-edu.tr
Fax: +90.312.2133921
