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Planta Med 2003; 69(4): 332-336
DOI: 10.1055/s-2003-38878
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Stimulatory Effect of Paeoniflorin on Adenosine Release to Increase the Glucose Uptake into White Adipocytes of Wistar Rat

Lap-Ming Tang1 , I-Min Liu2, 3 , Juei-Tang Cheng3
  • 1Department of Emergency Medicine, Mackay Memorial Hospital, Taipei City, Taiwan, R.O.C.
  • 2Department of Pharmacy, Tajen Institute of Technology, Yen-Pou, Ping Tung Shien, Taiwan, R.O.C.
  • 3Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R.O.C.
Further Information

Publication History

Received: July 5, 2002

Accepted: December 7, 2002

Publication Date:
23 April 2003 (online)

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Abstract

The present study investigated the role of adenosine in the stimulatory action of paeoniflorin on in vitro glucose transport. Paeoniflorin increased the uptake of a radiolabeled, non-metabolizable glucose derivative into isolated white adipocytes of Wistar rat in a concentration-dependent manner and this action was abolished by the antagonist, 8-cyclopentyltheophylline, at concentrations sufficient to block the adenosine A1 receptor. However, paeoniflorin failed to displace the binding of [3 H]-8-cyclopentyl-1,3-dipropylxanthine in the isolated cerebrocortex of Wistar rat. Direct activation of the adenosine A1 receptor does not seem to be responsible for the action of paeoniflorin. The stimulatory effect of paeoniflorin on radioactive glucose uptake was abolished in isolated rat white adipocytes pre-incubated with the adenosine deaminase at concentrations sufficient to metabolize endogenous adenosine. Mediation of endogenous adenosine in the action of paeoniflorin was further supported by the assay of adenosine released into the medium from rat white adipocytes incubated with paeoniflorin. These findings suggest that paeoniflorin could induce the release of adenosine from isolated rat white adipocytes and the released adenosine may activate the adenosine A1 receptor to enhance glucose uptake.

Key words

Paeoniflorin - Paeonia lactiflora - Ranunculaceae - adenosine A1 receptor - adipocytes - 8-cyclopentyltheophylline, [3 H]-8-cyclopentyl-1,3-dipropylxanthine - adenosine deaminase - adenosine

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Prof. Juei-Tang Cheng

Department of Pharmacology, College of Medicine

National Cheng Kung University

Tainan City

Taiwan 70101

R.O.C.

Phone: +886-6-237-2706

Fax: +886-6-238-6548

Email: jtcheng@mail.ncku.edu.tw