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Planta Med 2003; 69(4): 327-331
DOI: 10.1055/s-2003-38884
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

A New in vitro Tissue Factor Inhibitory Triterpene from the Fruits of Chaenomeles sinensis

Ming Hong Lee1 , Yong Nam Han1, 2
  • 1Natural Products Research Institute, Seoul National University, Seoul, Korea
  • 2College of Pharmacy, Seoul National University, Seoul, Korea
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Publikationsverlauf

Received: July 28, 2002

Accepted: January 4, 2003

Publikationsdatum:
23. April 2003 (online)

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Abstract

Tissue factor (TF, tissue thromboplastin) accelerates the blood clotting, activating both the intrinsic and the extrinsic pathways to serve as a cofactor. In order to isolate TF inhibitors from the fruits of Chaenomeles sinensis, an activity-guided purification was carried out to yield four triterpenoid compounds. One compound was new and its structure was elucidated as 28-O-β-D-glucopyranosyl-2α,3β-dihydroxyolean-12-ene-24,28-dioic acid (2), named chaenomeloside A by means of spectral analysis and chemical conversion. Other compounds were trachelosperoside A-1 (1), oleanolic acid (3) and ursolic acid (4). Compound 2 and its aglycone 2a, named chaenomelogenin A inhibited by 50 % the TF activity at concentrations of 0.036 and 0.028 mM/unit of TF, respectively. Compound 1 isolated for the first time from this plant as well as 3 and 4 were inactive.

Key words

Chaenomeles sinensis - Rosaceae - 28-O-β-D-glucopyranosyl-2α,3β-dihydroxyolean-12-ene-24,28-dioic acid - chaenomeloside A - chaenomelogenin A - trachelosperoside A-1 - tissue factor inhibitor

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Prof. Dr. Yong Nam Han

Natural Products Research Institute

Seoul National University

Seoul 110-460

Korea

eMail: snake@snu.ac.kr

Fax: +82-2-762-8322