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DOI: 10.1055/s-2003-38887
© Georg Thieme Verlag Stuttgart · New York
Effects of Baicalin on the Gene Expression of Surfactant Protein A (SP-A) in Lung Adenocarcinoma Cell Line H441
Publication History
Received: July 30, 2002
Accepted: January 4, 2003
Publication Date:
23 April 2003 (online)
![](https://www.thieme-connect.de/media/plantamedica/200304/lookinside/thumbnails/10.1055-s-2003-38887-1.jpg)
Abstract
Pulmonary surfactant, a complex lipoprotein, is secreted by alveolar type II cells. It lies at the alveolar air-fluid interface and prevents alveolar collapse by reducing surface tension. The high incidence of respiratory distress syndrome (RDS) in premature infants results principally from a deficiency of pulmonary surfactant. Surfactant protein A (SP-A) is the most abundant surfactant protein and reduces surface tension at the alveolar air-liquid interface in lung cells. In this study, RT-PCR and Western blot analyses of SP-A were performed to evaluate the biological activity of baicalin, a Chinese medicine prescribed extensively for preventing miscarriage. In in vitro experiments, lung adenocarcinoma cell line H441 was cultured with baicalin in varying concentrations and for varying lengths of time. The results show that the expression of SP-A gene was positively affected by baicalin in dose-dependent and time-course manners. The maximal expression of the SP-A gene, 1.7-fold greater than control, is induced at 150 nM of baicalin treated for 48 h.
Abbreviations
SP:surfactant protein
RDS:respiratory distress syndrome
IFN-γ:insulin, interferon gamma
TGF-β1:transforming growth factor β1
db-cAMP:dibutyryl adenosine 3′,5′-monophosphate
RT-PCR:reverse transcription-polymerase chain reaction
Key words
Scutellaria baicalensis - Lamiaceae - surfactant protein A - baicalin - traditional Chinese medicine - gene expression
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Dr. Kur-Ta Cheng
Department of Biochemistry
Taipei Medical University
No. 250, Wu-Hsing St.
Taipei 110
Taiwan
R. O. C.
Email: ktbot@tmu.edu.tw
Fax: +886-2-27399650