Separation and Determination of Coumarins in the Root Bark of Three Citrus Plants by Micellar Electrokinetic Capillary Chromatography

Shu Fang Wang; Yong Ju; Xing Guo Chen; Zhi De Hu

doi:10.1055/s-2003-39697

Planta Medica, Table of Contents

Planta Med 2003; 69(5): 483-486
DOI: 10.1055/s-2003-39697

Letter

Separation and Determination of Coumarins in the Root Bark of Three Citrus Plants by Micellar Electrokinetic Capillary Chromatography

Shu Fang Wang² , Yong Ju^1, ³ , Xing Guo Chen² , Zhi De Hu²

¹National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, P. R. China
²Department of Chemistry, Lanzhou University, Lanzhou, P. R. China
³The Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing, P. R. China

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the determination of xanthyletin and xanthoxyletin in the roots bark of three Citrus plants was developed for the first time. The electrophoresis buffer was 10 mmol/L sodium borate containing 50 mmol/L sodium dodecyl sulfate, 20 % (v/v) acetonitrile and 10 % (v/v) methanol (pH 9.8). The correlation coefficients of the calibration curves for xanthyletin and xanthoxyletin are 0.9993 and 0.9999, respectively, over the concentration ranges (16.0 - 800.0 mg/L; 8.0 - 400.0 mg/L).

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Prof. Xingguo Chen

Department of Chemistry

Lanzhou University

Lanzhou, 730000

P. R. China

Email: chenxg@lzu.edu.cn

Fax: +86-931-8912582