Antibodies to Heat-Shock Protein 65 and Neopterin Levels in Patients with Type 1 Diabetes Mellitus

R. Weitgasser; M. Lechleitner; T. Koch; G. Galvan; J. Mühlmann; K. Steiner; F. Hoppichler

doi:10.1055/s-2003-39784

Experimental and Clinical Endocrinology & Diabetes, Inhaltsverzeichnis

Exp Clin Endocrinol Diabetes 2003; 111(3): 127-131
DOI: 10.1055/s-2003-39784

Article

J. A. Barth Verlag in Georg Thieme Verlag Stuttgart · New York

Antibodies to Heat-Shock Protein 65 and Neopterin Levels in Patients with Type 1 Diabetes Mellitus

R. Weitgasser¹ , M. Lechleitner² , T. Koch² , G. Galvan¹ , J. Mühlmann³ , K. Steiner³ , F. Hoppichler² ^, ³

¹1st Department of Medicine, St.Johanns Spital, Salzburg General Hospital, Salzburg, Austria
²Department of General Internal Medicine, University of Innsbruck, Innsbruck, Austria
³Hospital Barmherzige Brüder, Salzburg, Austria

Abstract

Antibodies to heat shock protein (hsp) are strongly associated with atherosclerotic cardiovascular disease in the non-diabetic population as well as in patients with type 2 diabetes mellitus. In type 1 diabetes increased antibody titers to hsp were found to be a symptom of the autoimmune disease leading to beta-cell damage. We asked whether hsp antibody titers are related to metabolic control and late complications in type 1 diabetic patients. Serum neopterin, also an indicator of chronic inflammation, was also evaluated. The hsp65 antibody titer was determined in 138 patients with type 1 diabetes, 47 women and 91 men, aged 35.5 ± 12 years with a mean diabetes duration of 16.6 ± 10.5 years. A history of diabetic late complications and cardiovascular disease was taken. A fundoscopy and a neurological examination were performed, nephropathy was assessed by measurement of the urinary albumin excretion rate. For the measurement of the hsp antibody titer an enzyme-linked immunosorbent assay (ELISA) was applied, for neopterin a radio-immuno assay (RIA) was used. The hsp65 antibody titer was found to be positively related to the patients' age (r = 0.237; p < 0.035). Patients with retinopathy revealed significantly higher hsp65 antibody titers (307.2 ± 38.6) than those without retinopathy (150.0 ± 18.5;p < 0.003). No correlation was found between hsp antibody titer and metabolic control. Serum neopterin levels revealed a trend towards a positive relationship with diabetes duration (r = 0.205; p < 0.0539) and a significant correlation with serum cholesterol levels (r = 0.436; p < 0.001), but not with HbA1 c values.

Our data add further information to the role of inflammatory markers in the development of diabetic microangiopathy.

Key words

Diabetes mellitus type 1 - heat shock protein 65 - neopterin - microvascular complications

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Friedrich Hoppichler

Hospital Barmherzige Brüder Salzburg,

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Austria

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