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J Reconstr Microsurg 2003; 19(4): 249-256
DOI: 10.1055/s-2003-40581
Copyright © 2003 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Extension of Composite Tissue Allograft Survival Across Major Histocompatibility Barrier Under Short Course of Anti-Lymphocyte Serum and Cyclosporine A Therapy

Kagan Ozer, Raffi Gurunluoglu, Maciej Zielinski, Dariusz Izycki, Murat Unsal, Maria Siemionow
  • Department of Plastic Surgery, The Cleveland Clinic Foundation, Cleveland, OH
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Publication History

Publication Date:
14 July 2003 (online)

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ABSTRACT

In this study, the authors investigated the effects of combined use of cyclosporine A (CsA) and anti-lymphocyte serum (ALS) on the survival of rat hindlimb allografts across a fully allogeneic major histocompatibility complex (MHC) barrier between Brown-Norway rats (BN, RT1n) and Lewis rats (LEW, RT1l). Thirty transplantations were performed in five groups of six rats each: Group 1 was the isograft control; Group 2 was the allograft control; Group 3 received ALS, Group 4 received CsA, and Group 5 received CsA and ALS. Treatment was started 2 hr before surgery and was then given for 21 days. Donor-derived chimerism was monitored by FACS analysis. Survival time was calculated as the number of post-transplant days until the first signs of rejection. The allografts in Group 2, Group 3, and Group 4 survived a mean of 5, 6, and 33 days, respectively. The longest mean survival time-51 days-was noted in Group 5 (p<0.05). Donor- derived chimerism peaked at 17 percent and fell to 0 percent at the time of rejection. A combined protocol of ALS/CsA extended survival of rat hindlimb allografts across a fully allogeneic MHC barrier.

KEYWORDS

Anti-lymphocyte serum - composite tissue allograft - cyclosporine - rat - hindlimb.

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