Induction of Apoptosis and G2/M Cell Cycle Arrest by Gleditsioside E from Gleditsia sinensis in HL-60 Cells

Lei Zhong; Guiqing Qu; Ping Li; Jian Han; Dean Guo

doi:10.1055/s-2003-40645

Planta Medica, Inhaltsverzeichnis

Planta Med 2003; 69(6): 561-563
DOI: 10.1055/s-2003-40645

Letter

Induction of Apoptosis and G₂/M Cell Cycle Arrest by Gleditsioside E from Gleditsia sinensis in HL-60 Cells

Lei Zhong¹ , Guiqing Qu¹ , Ping Li¹ , Jian Han¹ , Dean Guo¹

¹State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China

Abstract

Gleditsioside E, a triterpene saponin isolated from Gleditsia sinensis, showed significant cytotoxicity against Bel-7402, BGC-823, HeLa, HL-60 and MCF-7 cell lines. The results of flow cytometry with annexin V-FITC/PI double staining proved that gleditsioside E mainly induced early apoptosis in HL-60 cells. Gleditsioside E treatment resulted in a prominent increase of the G₂/M population in HL-60 cells, and an accumulation of the sub-G₁ (hypoploid) peak was observed.

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Referenzen

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Prof. Dr. Dean Guo

State Key Laboratory of Natural and Biomimetic Drugs

School of Pharmaceutical Sciences

Peking University

Xueyuan Road 38

Beijing 100083

P. R. China

eMail: gda@bjmu.edu.cn

Fax: +86-10-62092700