Effect of Carbohydrate Feeding During Recovery from Prolonged Running on Muscle Glycogen Metabolism During Subsequent Exercise

Abstract

This study examined the effect of carbohydrate (CHO) intake during a 4 h recovery from prolonged running on muscle glycogen metabolism during subsequent exercise. On 2 occasions, 7 male subjects ran for 90 min at 70 % maximum oxygen uptake (V˙O₂max) on a motorized treadmill (R1) followed by a 4 h rest period (REC) and a 15 min run (R2) consisting of 5 min at 60 % and 10 min at 70 % V˙O₂max. During REC, each subject ingested a total of 2.7 l of an isotonic solution containing either 50 g of CHO (LOW) or 175 g of CHO (HIGH). Biopsy samples were obtained from the vastus lateralis immediately after R1, REC and R2. During REC, a higher muscle glycogen resynthesis was observed in HIGH when compared with LOW trial (75 ± 20 vs. 31 ± 11 mmol × kg dry matter (dm)^-1, respectively; p < 0.01). Muscle glycogen utilization during R2 was similar between the HIGH and LOW trials (39 ± 10 vs. 46 ± 11 mmol × kg dm^-1, respectively). These results suggest that ingestion of a large amount of CHO at frequent intervals during recovery from exercise does not affect the rate of muscle glycogen utilization during subsequent exercise.

Carbohydrate feeding · exercise · muscle glycogen · recovery

References

• 1 Bergstrom J. Muscle electrolytes in man. Scan.  J Clin Lab Invest Suppl. 1962;  68 1-100
• 2 Bergstrom J, Hermansen L, Hultman E, Saltin B. Diet, muscle glycogen and physical performance.  Acta Physiol Scand. 1967;  71 140-150
• 3 Bilzon J LJ, Murphy J L, Allsopp A J, Wootton S A, Williams C. Influence of glucose ingestion by humans during recovery from exercise on substrate utilisation during subsequent exercise in a warm environment.  Eur J Appl Physiol. 2002;  87 318-326
• 4 Blom P SC, Hostmark A T, Vaage O, Kardel K K, Maehlum S. Effect of different post-exercise sugar diets on the rate of muscle glycogen synthesis.  Med Sci Sports Exerc. 1987;  19 491-496
• 5 Borg G AV. Perceived exertion: a note on "history" and methods.  Med Sci Sports Exerc. 1973;  5(2) 90-93
• 6 Casey A, Mann R, Banister K, Fox J, Morris P G, Macdonald I A, Greenhaff P L. Effect of carbohydrate ingestion on glycogen resynthesis in human liver and skeletal muscle, measured by ₁₃C MRS.  Am J Physiol. 2000;  278 E65-E75
• 7 Cederblad G, Carlin J I, Constantin-Teodosiu D, Harper P, Hultman E. Radioisotopic assays of CoASH and carnitine and their acetylated forms in human skeletal muscle.  Anal Biochem. 1990;  185 274-278
• 8 Constantin-Teodosiu D, Carlin J I, Cederblad G, Harris R C, Hultman E. Acetyl group accumulation and pyruvate dehydrogenase activity in human muscle during incremental exercise.  Acta Physiol Scand. 1991;  143 367-372
• 9 Constantin-Teodosiu D, Cederblad G, Hultman E. PDC activity and acetyl group accumulation in skeletal muscle during prolonged exercise.  J Appl Physiol. 1992;  73 2403-2407
• 10 Costill D L, Coyle E, Dalsky G, Evans W, Fink W, Hoopes D. Effects of elevated plasma FFA and insulin on muscle glycogen usage during exercise.  J Appl Physiol. 1977;  43(4) 695-699
• 11 Coyle E F, Coggan A R, Hemmert M K, Lowe R C, Walters T J. Substrate usage during prolonged exercise following a preexercise meal.  J Appl Physiol. 1985;  59 429-433
• 12 Doyle J A, Sherman W M, Strauss R L. Effect of eccentric and concentric exercise on muscle glycogen replenishment.  J Appl Physiol. 1993;  74 1848-1855
• 13 Fallowfield J, Williams C, Singh R. The influence of ingesting a carbohydrate-electrolyte beverage during 4 hours of recovery on subsequent endurance capacity.  Int J Sports Nutr. 1995;  5 285-299
• 14 Fallowfield J, Williams C. The influence of a high carbohydrate intake during recovery from prolonged, constant pace running.  Int J Sports Nutr. 1997;  7 10-25
• 15 Harris R C, Hultman E, Nordesjo L O. Glycogen, glycogenolytic intermediates and high energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values.  Scand J Clin Lab Invest. 1974;  33 109-120
• 16 Hawley J A, Palmer G, Noakes T D. Effect of 3 days of carbohydrate supplementation on muscle glycogen content and utilisation during a 1-h cycling performance.  Eur J Appl Physiol. 1997;  75 407-412
• 17 Hermansen L, Hultman E, Saltin B. Muscle glycogen during prolonged severe exercise.  Acta Physiol Scand. 1967;  71 129-139
• 18 Horowitz J F, Mora-Rodriguez R, Byerley L O, Coyle E F. Lipolytic suppression following carbohydrate ingestion limits fat oxidation during exercise.  Am J Physiol. 1997;  273 E768-E775
• 19 Hultman E, Nilsson L H. Liver glycogen in man. Effect of different diets and muscular exercise. In: Pernow B, Saltin B (eds) Muscle metabolism during exercise New York; Plenum Press 1971: 143-151
• 20 Jansson E. Acid soluble and insoluble glycogen in human skeletal muscle.  Acta Physiol Scand. 1981;  113 337-340
• 21 Ivy J L, Katz A L, Cutler C L, Sherman W M, Coyle E F. Muscle glycogen resynthesis after exercise: effect of time of carbohydrate ingestion.  J Appl Physiol. 1988a ;  64 1480-1485
• 22 Ivy J L, Le M C, Brozinick J T, Reed M J. Muscle glycogen storage after different amounts of carbohydrate ingestion.  J Appl Physiol. 1988;  65 2018-2023
• 23 Lowry O H, Passonneau J V. A Flexible System of Enzymatic Analysis. New York; Academic Press 1972: 198-193
• 24 Madsen K, Pedersen P K, Rose P, Richter E A. Carbohydrate supercompensation and muscle glycogen utilization during exhaustive running in highly trained athletes.  Eur J Appl Physiol. 1990;  61 467-472
• 25 Ren J M, Hultman E. Regulation of glycogenolysis in human skeletal muscle.  J Appl Physiol. 1989;  67 2243-2248
• 26 Taylor H R, Buskirk E R, Henschell A. Maximum oxygen uptake as an objective measure of cardio-respiratory performance.  J Appl Physiol. 1955;  8 73-80
• 27 Tsintzas O K, Williams C, Boobis L, Greenhaff P. Carbohydrate ingestion and single muscle fiber glycogen metabolism during prolonged running in men.  J Appl Physiol. 1996;  81 801-809
• 28 Wong S H, Williams C. Influence of different amounts of carbohydrate on endurance running capacity following short term recovery.  Int J Sports Med. 2000;  21 444-452