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Aktuelle Urol 2003; 34(4): 270-272
DOI: 10.1055/s-2003-41615
Original Article
© Georg Thieme Verlag Stuttgart · New York

Detection of Circulating MN/CA9 Positive Renal Cell Carcinoma Cells During Operation

Intraoperative Detektion von MN/CA9-positiven Nierenzellenkarzinom-ZellenH.  Uemura1 , Y.  Nakagawa1 , A.  Iwai1 , E.  Okajima1 , E.  Okajima1 , K.  Yoshikawa2 , Y.  Hirao1
  • 1Department of Urology, Nara Medical University, Nara, Japan
  • 2Second Department of Pathology, Aichi Medical University, Aichi, Japan
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Publication History

Publication Date:
25 August 2003 (online)

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Abstract

Our previous study demonstrated the clear detection of MN/CA9 mRNA in peripheral blood samples from RCC patients. However, approximately 30 % of control blood samples from healthy volunteers showed MN/CA9 expression. We have developed a new primer set and optimized the PCR conditions, now resulting in a specificity of 100 %. In tissue samples, all clear cell type carcinomas but none of the spindle cell type and pleomorphic cell type tumors expressed the MN/CA9 message. Analysis of MN/CA9 messages in peripheral blood samples from RCC patients gave positive results for 0/2, 1/9, 0/4 and 4/12 of stage I, II, III and IV cases, respectively. RT-PCR analysis using preoperative renal venous blood samples resulted in clear detection of MN/CA9 positive cells in 2/4, 3/13, 2/6 and 1/1 of stage I, II, III and IV cases, respectively. Our results suggest that assessment of MN/CA9 expression by RT-PCR is a promising method for detecting cancer cells in the circulation of patients with RCC.

Key words

Renal cell carcinoma - MN/CA9 - circulating cancer cells

References

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Hirotsugu Uemura, M. D. 

Department of Urology · Nara Medical University

840 Shijo-cho

Kashihara

Nara 634-8522

Japan

Phone: +81-744-29-8893

Fax: +81-744-22-9282

Email: huemura@naramed-u.ac.jp