Immune and Oxidative Changes During and Following the Western States Endurance Run

D. C. Nieman; C. I. Dumke; D. A. Henson; S. R. McAnulty; L. S. McAnulty; R. H. Lind; J. D. Morrow

doi:10.1055/s-2003-42018

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 2003; 24(7): 541-547
DOI: 10.1055/s-2003-42018

Immunology

Immune and Oxidative Changes During and Following the Western States Endurance Run

D. C. Nieman¹ , C. I. Dumke¹ , D. A. Henson¹ , S. R. McAnulty¹ , L. S. McAnulty¹ , R. H. Lind¹ , J. D. Morrow²

¹Departments of Health, Leisure, and Exercise Science; Biology; Family and Consumer Sciences; Appalachian State University, Boone, North Carolina, USA
²Departments of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennesse, USA

Abstract

Changes in immune and oxidative stress parameters were measured in ultramarathon runners competing in the 160-km Western States Endurance Run. Forty-five runners agreed to provide blood and saliva samples the morning before the race event, at the 90-km aid station, and 5 - 10 min post-race. Upper respiratory tract infection (URTI) during the two-week period post-race was assessed retrospectively by telephone interviews. Forty subjects completed 90-km (race time, 13.1 ± 0.3 h), and 31 completed the 160-km race event (27.0 ± 0.4 h). The blood neutrophil and monocyte counts rose 249 % and 214 %, respectively, in the 31 finishers. Salivary IgA (sIgA) secretion rate decreased significantly from 508 ± 40 µg/min pre-race, to 287 ± 39 µg/min at 90-km, and 254 ± 30 µg/min post-race (50 % decrease). Significant increases were measured in cytokines at 90-km and post-race, with post-race IL-10 increasing 9.5-fold, IL-1ra 6.1-fold, IL-6 50.2-fold, and IL-8 2.5-fold over pre-race levels. Post-race indicators of oxidative stress, F₂-isoprostane and lipid hydroperoxides, increased 33 % and 88 %, respectively. Pearson product-moment correlations revealed positive correlations at 90-km between F₂-isoprostane and IL-6 (r = 0.31, p = 0.048), IL-10 (r = 0.31, p = 0.050), and IL-8 (r = 0.43, p = 0.005), but no other significant relationships between immune and oxidative stress indicators at 90-km and post-race. In the group of runners completing at least 90 km of the race, 26 % reported an URTI episode during the two-week period post-race. A low sIgA secretion rate at 90-km was the best predictor of post-race URTI (173 ± 34 µg/min in those who later acquired URTI compared to 325 ± 40 µg/min in those without URTI, p = 0.007). In conclusion, a modest correlation was found between cytokines and F₂-isoprostane at 90-km when the greatest oxidative stress occurred, but no other significant correlations in immune and oxidative stress indicators during and following a 160-km ultramarathon race event were noted. About one in four ultramarathoners reported URTI during the two-week period post-race, and a low sIgA secretion rate mid-race best predicted URTI occurrence.

Key words

Oxidative stress - salivary IgA - upper respiratory tract infection - isoprostanes - cytokines

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References

1 Allesio H, Hagerman A E, Fulkerson B K, Ambrose J, Rice R E, Wiley R L. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000; 32 1576-1581
2 Askew E. Work at high altitude and oxidative stress: antioxidant nutrients. Toxicology. 2002; 180 107-119
3 Azenabor A A, Hoffman-Goetz L. Intrathymic and intrasplenic oxidative stress mediates thymocyte and splenocyte damage in acutely exercised mice. J Appl Physiol. 1999; 86 1823-1827
4 Benzie I FF, Strain J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem. 1996; 239 70-76
5 Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72 248-254
6 de la Fuente M. Effects of antioxidants on immune system aging. Eur J Clin Nutr. 2002; 56 5-8
7 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol. 1974; 37 247-248
8 Esposito K, Nappo F, Marfella R, Giugliano G, Giugliano F, Ciotola M, Quagliaro L, Ceriello A, Giugliano D. Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation. 2002; 106 2067-2072
9 Gleeson M, Hall S T, McDonald W A, Flanagan A J, Clancy R L. Salivary IgA subclasses and infection risk in elite swimmers. Immunol Cell Biol. 1999; 77 351-355
10 Gleeson M, McDonald W A, Pyne D B, Clancy R L, Cripps A W, Francis J L, Fricker P A. Immune status and respiratory illness for elite swimmers during a 12-week training cycle. Int J Sports Med. 2000; 21 302-307
11 Haddad J J, Fahlman C S. Redox- and oxidant-mediated regulation of interleukin-10: an anti-inflammatory, antioxidant cytokine. Biochem Biophys Res Commun. 2002; 297 163-176
12 Ji L L. Exercise-induced modulation of antioxidant defense. Ann N Y Acad Sci. 2002; 959 82-92
13 Mastaloudis A, Leonard S W, Traber M G. Oxidative stress in athletes during extreme endurance exercise. Free Radic Biol Med. 2001; 31 911-922
14 McAnulty S R, McAnulty L S, Nieman D C, Dumke C, Utter A C, Morrow J D, Henson D A, Proulx W R, George G L. Effect of blueberry phytochemicals and vitamin C on oxidative stress and immune changes following exercise in the heat. Free Radic Res. in press
15 Mooren F, Lechtermann A, Fromme A, Thorwesten L, Volker K. Alterations in intracellular calcium signaling of lymphocytes after exhaustive exercise. Med Sci Sports Exerc. 2001; 33 242-248
16 Morrow J D, Roberts L J. The isoprostanes: Unique bioactive products of lipid peroxidation. Prog Lipid Res. 1997; 36 1-21
17 Nieman D C. Immune response to heavy exertion. J Appl Physiol. 1997; 82 1385-1394.
18 Nieman D C, Henson D A, Fagoaga O R, Utter A C, Vinci D M, Davis J M, Nehlsen-Cannarella S L. Change in salivary IgA following a competitive marathon race. Int J Sports Med. 2002; 23 69-75
19 Nieman D C, Henson D A, McAnulty S R, McAnulty L, Swick N S, Utter A C, Vinci D M, Opiela S J, Morrow J D. Influence of vitamin C supplementation on oxidative and immune changes following an ultramarathon. J Appl Physiol. 2002; 92 1970-1977
20 Nieman D C, Henson D A, Smith L L, Utter A C, Vinci D M, Davis J M, Kaminsky D E, Shute M. Cytokine changes after a marathon race. J Appl Physiol. 2001; 91 109-114
21 Nieman D C, Johansen L M, Lee J W, Cermak J, Arabatzis K. Infectious episodes in runners before and after the Los Angeles Marathon. J Sports Med Phys Fit. 1990; 30 316-328
22 Nieman D C, Peters E M, Henson D A, Nevines E I, Thompson M M. Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res. 2000; 20 1029-1035
23 Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen B K. Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans. J Physiol. 1999; 515(Pt 1) 287-291
24 Ostrowski K, Schjerling P, Pedersen B K. Physical activity and plasma interleukin-6 in humans - effect of intensity of exercise. Eur J Appl Physiol. 2000; 83 512-515
25 Pistilli E E, Nieman D C, Henson D A, Kaminsky D E, Utter A C, Vinci D M, Davis J M, Fagoaga O R, Nehlsen-Cannarella S L. Influence of age on immune changes in runners after a marathon. J Aging Physical Activity. 2002; 10 432-442
26 Roberts L J, Morrow J D. Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation. Cell Mol Life Sci. 2002; 59 808-820
27 Schmidt M C, Askew E W, Roberts D E, Prior R L, Ensign W Y, Hesslink R E. Oxidative stress in humans training in a cold, moderate altitude environment and their response to a phytochemical antioxidant supplement. Wilderness Environ Med. 2002; 13 94-105
28 Sofic E, Rustembegovic A, Kroyer G, Cao G. Serum antioxidant capacity in neurological, psychiatric, renal diseases and cardiomyopathy. J Neural Transm. 2002; 109 711-719
29 Splettstoesser W D, Schuff-Werner P. Oxidative stress in phagocytes - ”the enemy within“. Microsc Res Tech. 2002; 57 441-455
30 Steerenberg P A, van Asperen I A, van Nieuw Amerongen A, Biewenga A, Mol D, Medema G J. Salivary levels of immunoglobulin A in triathletes. Eur J Oral Sci. 1997; 105 305-309
31 Suzuki K, Yamada M, Kurakake S, Okamura N, Yamaya K, Liu Q, Kudoh S, Kowatari K, Nakaji S, Sugawara K. Circulating cytokines and hormones with immunosuppressive but neutrophil-priming potentials rise after endurance exercise in humans. Eur J Appl Physiol. 2000; 81 281-287
32 Tomasi T B, Trudeau F B, Czerwinski D, Erredge S. Immune parameters in athletes before and after strenuous exercise. J Clin Immunol. 1982; 2 173-178
33 Vider J, Lehtmaa J, Kullisaar T, Vihalemm T, Zilmer K, Kairane C, Landor A, Karu T, Zilmer M. Acute immune response in respect to exercise-induced oxidative stress. Pathophysiology. 2001; 7 263-270
34 Vina J, Gomez-Cabrera M, Lloret A, Marquez R, Minana J B, Pallardo F V, Sastre J. Free radicals in exhaustive exercise: Mechanisms of production, and protection by antioxidants. IUBMB Life. 2000; 50 271-277

D. C. Nieman, Dr.PH, FACSM

Department of Health & Exercise Science

Appalachian State University · Boone · North Carolina 28608 · USA

Telefon: +1 828 262 6318

Fax: +1 828 262 3138

eMail: niemandc@appstate.edu