Subscribe to RSS
DOI: 10.1055/s-2003-45145
© Georg Thieme Verlag Stuttgart · New York
Antinociceptive Effects of Ginsenosides Injected Intracerebroventricularly or Intrathecally in Substance P-Induced Pain Model
This work was supported by the Research Grant from Hallym University, South KoreaPublication History
Received: April 8, 2003
Accepted: August 24, 2003
Publication Date:
09 January 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200311/lookinside/thumbnails/10.1055-s-2003-45145-1.jpg)
Abstract
We have examined the effects of several ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 and Rg3) adminstered intracerebroventricularly (i. c. v.) or intrathecally (i. t.) on the nociceptive behavior induced by substance P (0.7 μg) injected i. t. Among the several ginsenosides studied, Rb2, Rc, Rd, and Re, but not Rb1, Rf, Rg1 and Rg3, treated i. c. v. (50 μg) attenuated the nociceptive behavior induced by substance P injected i. t. On the other hand, we found that i. t. treatment with 50 μg of Rb1, Rb2, Rd, or Rf effectively attenuated the nociceptive behavior induced by i. t. injected substance P. However, the i. t. treatment with the same doses of Rc, Re, Rg1 or Rg3 was not effective for antagonizing i. t. injected substance P-induced nociceptive behavior. Our results show that ginsenosides Rb2, Rc, Rd, or Re injected supraspinally exert a antinociceptive effect in the substance P-induced pain model. Furthermore, Rb1, Rb2, Rd, or Rf treated spinally produce antinociception in the substance P-induced pain model.
Abbreviations
i.c.v.:intracerebroventricular
i.t.:intrathecal
S.E.M.:Standard error of the means
Key Words
Ginsenosides - Panax ginseng C.A. Meyer - Araliaceae - antinociception - supraspinal - spinally - substance P pain model - mouse
References
- 1 Kim H S, Oh K W, Rheu H M, Kim S R. Antagonism of U-50,488H-induced antinociception by ginseng total saponins is dependent on serotonergic mechanisms. Pharmacol Biochem Behav. 1992; 42 587-93
- 2 Suh H W, Song D K, Huh S O, Kim Y R. Modulatory role of ginsenosides injected intrathecally or intracerebroventricularly in the production of antinociception induced by kappa-opioid receptor agonist administered intracerebroventricularly in the mouse. Planta Med. 2000; 66 412-7
- 3 Suh H W, Song D K, Kim Y R. Effects of ginsenosides injected intrathecally or intracerebroventricularly on antinociception induced by morphine administrated intracerebroventricularly in the mouse. Gen Pharmacol. 1997; 29 873-7
- 4 Shin Y R, Jung O M, Nah J J, Nam K Y, Kim C Y, Nah S Y. Ginsenosides that produce differential antinociception in mice. Gen Pharmacol. 1999; 32 53-9
- 5 Hylden J LK, Wilcox G L. Intrathecal substance P elicits a caudally-directed biting and scratching behavior in mice. Brain Res. 1981; 217 212-5
- 6 Hunskaar S, Rasmer O B, Hole K. Acetylsalicylic acid, paracetamol and morphine inhibit behavioral responses to intrathecally administered substance P or capsaicin. Life Sci. 1998; 37 835-41
- 7 Haley T J, McCormick W G. Pharmacological effects produced by intracerebral injections of drugs in the conscious mouse. Br J Pharmacol. 1957; 12 12-5
- 8 Hylden J LK, Wilcox G L. Intrathecal morphine in mice: a new technique. Eur J Pharmacol. 1980; 67 313-6
- 9 Dunham N W, Miya T S. A note on a simple apparatus for detecting neurological deficit in rats and mice. J Am Pharm Assoc. 1957; 46 208-9
- 10 Nah J J, Hahn J H, Chung S, Choi S, Kim Y I, Nah S Y. Effect of ginsenosides, active components of ginseng, on capsaicin-induced pain-related behavior. Neuropharmacology. 2000; 39 2180-4
- 11 Yoon S R, Nah J J, Shin Y R, Kim S K, Nam K Y, Choi H S, Nah S Y. Ginsenosides induce differential antinociception and inhibit substance P induced-nociceptive response in mice. Life Sci. 1998; 62 PL319-25
- 12 Mogil J S, Shin Y R, McCleskey E W, Kim S C, Nah S Y. Ginsenoside Rf, a trace component of ginseng root, produces antinociception in mice. Brain Res. 1998; 792 218-28
- 13 Nemmani K V, Ramarao P. Role of benzodiazepine-GABAA receptor complex in attenuation of U-50,488H-induced analgesia and inhibition of tolerance to its analgesia by ginseng total saponin in mice. Life Sci. 2002; 70 1727-40
Professor Hong-Won Suh, Ph. D.
Department of Pharmacology
College of Medicine
Hallym University
1 Okchun-dong
Chunchon
Kangwon Do 200-702
South Korea
Phone: +82-33-248-2614
Fax: +82-33-248-2612
Email: hwsuh@hallym.ac.kr