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DOI: 10.1055/s-2003-45155
Cytotoxic Triterpenoids from the Fruits of Zizyphus jujuba
This research was supported by a grant (code PF002107-00) from Plant Diversity Research Center of 21st Frontier Research Program funded by Ministry of Science and Technology of Korean GovernmentPublication History
Received: May 7, 2003
Accepted: August 2, 2003
Publication Date:
09 January 2004 (online)
Abstract
The following eleven triterpenoic acids were isolated from the fruits of Zizyphus jujuba (Rhamnaceae): colubrinic acid, alphitolic acid, 3-O-cis-p-coumaroylalphitolic acid (3), 3-O-trans-p-coumaroylalphitolic acid (4), 3-O-cis-p-coumaroylmaslinic acid, 3-O-trans-p-coumaroylmaslinic acid, betulinic acid (7), oleanolic acid, betulonic acid (9), oleanonic acid and zizyberenalic acid. The in vitro cytotoxicities of the triterpenoic acids against K562, B16(F-10), SK-MEL-2, PC-3, LOX-IMVI, and A549 tumor cell lines were investigated by the sulforhodamin B (SRB) method. Among these compounds, the lupane-type triterpenes, such as compounds 3, 4, 7, and 9, showed high cytotoxic activities. In particular, the cytotoxic activities of 3-O-p-coumaroylalphitolic acids (compounds 3 and 4) were better than those of non-coumaroic triterpenenoids (compounds 7 and 9). These results suggest that the coumaroyl moiety at the C-3 position of the lupane-type triterpene may play an important role in enhancing cytotoxic activity.
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Dr. Yung Hee Kho
Korea Research Institute of Bioscience and Biotechnology
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