Planta Med 2003; 69(12): 1172-1174
DOI: 10.1055/s-2003-818015
Letter
© Georg Thieme Verlag Stuttgart · New York

DNA Microarray for Identification of the Herb of Dendrobium Species from Chinese Medicinal Formulations

Yan-Bo Zhang1 , Jun Wang1 , 3 , Zheng-Tao Wang4 , Paul Pui-Hay But2 , 3 , Pang-Chui Shaw1 , 3
  • 1Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  • 2Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  • 3Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  • 4Department of Pharmacognosy, Chinese Pharmaceutical University, Nanjing, China
Further Information

Publication History

Received: June 23, 2003

Accepted: September 7, 2003

Publication Date:
29 January 2004 (online)

Abstract

A DNA microarray for detecting processed medicinal Dendrobium species (Herba Dendrobii) was constructed by incorporating the ITS1-5.8S-ITS2 sequences of 16 Dendrobium species on a glass slide. Using fluorescence-labeled ITS2 sequences as probes, distinctive signals were obtained for the five medicinal Dendrobium species listed in the Chinese Pharmacopoeia. The established microarray was able to detect the presence of D. nobile in a Chinese medicinal formulation containing nine herbal components.

References

  • 1 Chinese Pharmacopoeia Editorial Committee.  Chinese Pharmacopoeia. Vol. 1 Chemical Technology Press Bejing; 2000: p. 70
  • 2 Li M F, Xu G J, Ma G X, Jin R L. Survey and identification of Herba Dendrobii.  Chinese Traditional and Herbal Drugs. 1986;  17 465-8
  • 3 Li M F, Xu G J, Ma G X, Jin R L. Survey and identification of Herba Dendrobii (II).  Chinese Traditional and Herbal Drugs. 1991;  22 173-6
  • 4 Ma G X, Xu G J, Li M F. Survey and identification of Herba Dendrobii (III).  Chinese Traditional and Herbal Drugs. 1995;  26 370
  • 5 Lau D TW, Shaw P C, Wang J, But P PH. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA.  Planta Medica. 2001;  67 456-60
  • 6 Trau D, Lee T MH, Lao A LK, Lenigk R, Hsing I M, IP N Y, Carles M C, Sucher N J. Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray.  Analytical Chemistry. 2002;  74 3168-73
  • 7 Yang Z, Zhang Y O, Huang W X, Xiao P G, Yang M S. Application of genomics and biochip technology in research and development of Chinese material medica.  Acta Pharmaceutica Sinica. 2002;  37 490-6
  • 8 Liu Y P, Hui C, Komatsu K, But P PH. Quality control for Chinese herbal drugs using DNA probe technology.  Acta Pharmaceutica Sinica. 2001;  36 475-80

Prof. P C. Shaw

Department of Biochemistry

The Chinese University of Hong Kong

Shatin, N.T.

Hong Kong

P. R. China

Phone: +852-2609-6803

Fax: +852-2603-5123

Email: pcshaw@cuhk.edu.hk