Abstract
A DNA microarray for detecting processed medicinal Dendrobium species (Herba Dendrobii) was constructed by incorporating the ITS1-5.8S-ITS2 sequences of 16 Dendrobium species on a glass slide. Using fluorescence-labeled ITS2 sequences as probes, distinctive signals were obtained for the five medicinal Dendrobium species listed in the Chinese Pharmacopoeia. The established microarray was able to detect the presence of D. nobile in a Chinese medicinal formulation containing nine herbal components.
References
-
1 Chinese Pharmacopoeia Editorial Committee. Chinese Pharmacopoeia. Vol. 1 Chemical Technology Press Bejing; 2000: p. 70
-
2
Li M F, Xu G J, Ma G X, Jin R L.
Survey and identification of Herba Dendrobii.
Chinese Traditional and Herbal Drugs.
1986;
17
465-8
-
3
Li M F, Xu G J, Ma G X, Jin R L.
Survey and identification of Herba Dendrobii (II).
Chinese Traditional and Herbal Drugs.
1991;
22
173-6
-
4
Ma G X, Xu G J, Li M F.
Survey and identification of Herba Dendrobii (III).
Chinese Traditional and Herbal Drugs.
1995;
26
370
-
5
Lau D TW, Shaw P C, Wang J, But P PH.
Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA.
Planta Medica.
2001;
67
456-60
-
6
Trau D, Lee T MH, Lao A LK, Lenigk R, Hsing I M, IP N Y, Carles M C, Sucher N J.
Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray.
Analytical Chemistry.
2002;
74
3168-73
-
7
Yang Z, Zhang Y O, Huang W X, Xiao P G, Yang M S.
Application of genomics and biochip technology in research and development of Chinese material medica.
Acta Pharmaceutica Sinica.
2002;
37
490-6
-
8
Liu Y P, Hui C, Komatsu K, But P PH.
Quality control for Chinese herbal drugs using DNA probe technology.
Acta Pharmaceutica Sinica.
2001;
36
475-80
Prof. P C. Shaw
Department of Biochemistry
The Chinese University of Hong Kong
Shatin, N.T.
Hong Kong
P. R. China
Phone: +852-2609-6803
Fax: +852-2603-5123
Email: pcshaw@cuhk.edu.hk
