Effects of Imidapril, an Angiotensin-converting Enzyme Inhibitor, on Insulin Sensitivity and Responsiveness in Streptozotocin-induced Diabetic Rats

A. Y. I. Viana; Y. Oshida; Y.-Q. Han; K. Koshinaka; Y. Sato

doi:10.1055/s-2004-814100

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2004; 36(1): 34-38
DOI: 10.1055/s-2004-814100

Original Clinical

Effects of Imidapril, an Angiotensin-converting Enzyme Inhibitor, on Insulin Sensitivity and Responsiveness in Streptozotocin-induced Diabetic Rats

A. Y. I. Viana¹ , Y. Oshida^1, ² , Y-Q. Han² , K. Koshinaka¹ , Y. Sato^1, ²

¹Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
²Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan

Abstract

We have studied the effect of imidapril, an angiotensin-converting enzyme inhibitor, on streptozotocin-induced diabetic rats. A sequential euglycemic hyperinsulinemic clamp procedure was used (insulin infusion rates: 3 and 30 mU/kg BW/min) in 30 diabetic rats. The rats were divided in 6 groups: a control group, a control group with N-monomethyl-L-arginine (L-NMMA, 1 mg/kg/min, a nitric oxide synthase inhibitor) infusion, a streptozotocin-induced diabetic group, a diabetic group with L-NMMA infusion, a diabetic group involving imidapril infusion (5 μg/kg/min), and a diabetic group involving simultaneous imidapril and L-NMMA infusion. Glucose concentrations were maintained around 140 mg/dl during the clamp studies. Plasma insulin levels during the 3 and 30 mU/kg BW/min insulin infusions were 30 and 400 μU/ml, respectively. Glucose infusion rates (GIR) in STZ-induced diabetic rats showed a significant decrease compared to controls. At both insulin infusion rates, imidapril-infused diabetic rats showed an increased GIR, compared with the saline infused ones. There was no significant difference in GIR between L-NMMA and saline infusion in diabetic rats. Simultaneous infusion of imidapril and L-NMMA did not significantly decrease GIR with low-dose insulin infusion, but the increase in GIR induced by imidapril with high-dose insulin infusion was impaired by 100 % by L-NMMA infusion in diabetic rats. These results suggest that imidapril may improve insulin action, in part, via nitric oxide.

Key words

Angiotensin-Converting Enzyme Inhibitor - Euglycemic Clamp Technique - L-NMMA - Streptozotocin

Full Text

References

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Y. Sato, M. D, Ph. D.

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