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Horm Metab Res 2004; 36(2): 101-106
DOI: 10.1055/s-2004-814219
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Contribution of Interleukin-12 to the Pathogenesis of Non-thyroidal Illness

A.  Boelen1 , J.  Kwakkel1 , M.  Platvoet-ter Schiphorst1 , A.  Baur2 , J.  Köhrle2 , W.  M.  Wiersinga1
  • 1Department of Endocrinology, Academic Medical Center, University of Amsterdam, The Netherlands
  • 2Klinische Forschergruppe, Medizinische Poliklinik der Universitaet Wuerzburg, Wuerzburg, and Inst. Exp. Endokrinologie, Charité, Humboldt-University, Berlin, Germany
Weitere Informationen

Publikationsverlauf

Received 25 March 2003

Accepted after revision 19 August 2003

Publikationsdatum:
05. März 2004 (online)

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Abstract

Changes in both central and peripheral thyroid hormone (TH) metabolism occur during illness. These changes, known collectively as non-thyroidal illness, are apparently mediated by the proinflammatory cytokines IL-6, TNFα and IFNγ. IL-12 is involved in regulation of IFNγ and TNFα. The aim of this study was to evaluate the role of IL-12 in TH metabolism during illness. We studied TH metabolism both centrally (in the pituitary) and peripherally (in the liver) in IL-12 knock-out (IL-12-/-) and wild type (WT) mice during illness induced by administration of bacterial endotoxin (LPS). LPS induced a similar decrease in serum T3, T4 and liver 5′-DI mRNA expression in IL-12-/- and WT mice with the exception of a smaller reduction of serum T4 in IL-12-/- mice. In the pituitary, the LPS-induced decline in 5′-DI activity in WT mice was not observed in IL-12-/- mice (p < 0.001), whereas the decrease in DII activity tended to be smaller in IL-12-/- mice (p = 0.066). The lower decrease in pituitary activity of both DI and DII in IL-12-/- mice is possibly related to the lower LPS-induced T4 decrease. In conclusion, IL-12 is involved in the central regulation of the HPT axis during illness but not in the peripheral regulation.

Key words

5′-deiodinase - Pituitary - Liver - Cytokines - IL-12-/- mice

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Dr. A. Boelen

Department of Endocrinology & Metabolism · F5-171, Academic Medical Center ·

Meibergdreef 9 · 1105 AZ Amsterdam · The Netherlands

Telefon: + 31 (20) 566 57 49 ·

eMail: a.boelen@amc.uva.nl