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Planta Med 2004; 70(1): 17-22
DOI: 10.1055/s-2004-815449
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Suppression of Infection-Induced Endotoxin Shock in Mice by a Citrus Flavanone Naringin

Kiichiro Kawaguchi1 , Sei-ichi Kikuchi1 , Ryoichi Hasunuma2 , Hiroko Maruyama3 , Roland Ryll2 , Yoshio Kumazawa2
  • 1Medicinal Plant Garden, School of Pharmaceutical Sciences, Kitasato University, Japan
  • 2Department of Biosciences, School of Science, Kitasato University, Japan
  • 3Department of Pathology, School of Allied Health Sciences, Kitasato University, Japan
This study was supported in part by grant-in-aid for Research on Health Sciences focusing on Drug from the Japan Health Sciences Foundation (KH31031) to Y.K., and a grant-in-aid for Scientific Research (Project 12 and 15) from the School of Pharmaceutical Sciences, Kitasato University to K.K.
Further Information

Publication History

Received: June 23, 2003

Accepted: November 22, 2003

Publication Date:
06 February 2004 (online)

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Abstract

The protective effect of the Citrus flavanone naringin was demonstrated in an endotoxin shock model based on Salmonella infection. Intraperitoneal (i. p.) infection with 108 CFU Salmonella typhimurium aroA caused lethal shock in lipopolysaccharide (LPS) -responder but not LPS-non-responder mice. Administration of 1 mg naringin 3 h before infection resulted in protection from lethal shock, similar to LPS-non-responder mice. The protective effect of naringin was time- and dose-dependent. Treatment with naringin resulted not only in a significant decrease in bacterial numbers in spleens and livers, but also in a decrease in plasma LPS levels. In addition, naringin markedly suppressed TNF-α and normalized the activated states of blood coagulation factors such as prothrombin time, fibrinogen concentration and platelet numbers caused by infection. Interestingly, treatment with naringin suppressed high levels of soluble CD14 and high mobility group-1 molecule caused by infection.

Key words

Salmonella-infection - endotoxin shock - flavanone - naringin - blood coagulation factors - high mobility group-1 molecule

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Dr. Yoshio Kumazawa

Department of Biosciences

School of Science

Kitasato University

1-15-1 Kitasato

Sagamihara

Kanagawa 228-8555

Japan

Phone: +81-42-778-9534

Fax: +81-42-778-9534

Email: kumazawa@jet.sci.kitasato-u.ac.jp