Planta Med 2004; 70(1): 45-49
DOI: 10.1055/s-2004-815454
Original Paper
Biochemistry and Molecular Biology
© Georg Thieme Verlag Stuttgart · New York

Rearranged Jatrophane-Type Diterpenes from Euphorbia Species. Evaluation of their Effects on the Reversal of Multidrug Resistance

Ana M. Madureira1 , Maria-José U. Ferreira1 , Nora Gyémánt4 , Katalin Ugocsai4 , José R. Ascenso2 , Pedro M. Abreu3 , Judit Hohmann5 , Joseph Molnár4
  • 1Centro de Estudos de Ciências Farmacêuticas, Faculdade de Farmácia da Universidade de Lisboa, Lisboa, Portugal
  • 2Centro de Química Estrutural, Instituto Superior Técnico, Lisboa, Portugal
  • 3CQFB/REQUIMTE, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, Caparica, Portugal
  • 4Department of Medical Microbiology, University of Szeged, Szeged, Hungary
  • 5Department of Pharmacognosy, University of Szeged, Szeged, Hungary
This work was supported by FCT (POCTI, Quadro Comunitário de Apoio III)
Further Information

Publication History

Received: June 6, 2003

Accepted: October 3, 2003

Publication Date:
06 February 2004 (online)

Abstract

The rearranged jatrophane-type diterpenes (1 - 3), isolated from the Me2CO extracts of Euphorbia portlandica and Euphorbia segetalis, were examined for their effects on multidrug resistance (MDR) in mouse lymphoma cells. Compounds 2 and 3 revealed to be active with the latter being more active than the positive control verapamil, a known resistance modifier. The new compound 1, named portlandicine, was isolated from Euphorbia portlandica and its structure characterised by high-field NMR spectroscopic methods including 2D NMR techniques: COSY, HMQC, HMBC and NOESY. The known diterpene 2, together with aleuritolic acid (4), oleanolic acid (5), and betulin diacetate (6), were also isolated from the same species.

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Prof. Maria José Umbelino Ferreira

Centro de Estudos de Ciências Farmacêuticas

Faculdade de Farmácia da Universidade de Lisboa

Av. das Forças Armadas

1600-083 Lisboa

Portugal

Fax: +351-21-7946470

Email: mjuferreira@ff.ul.pt