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DOI: 10.1055/s-2004-815501
A Secoisopimarane Diterpenoid from Salvia cinnabarina Inhibits Rat Urinary Bladder Contractility in vitro
This work was supported by Cofinanziamento Murst, Enrico and Enrica Sovena Foundation (Rome, Italy), Regione Campania. SR140333, SR48968 and S141716A were a kind gift from SANOFI (Montpellier, France)Publication History
Received: July 28, 2003
Accepted: October 3, 2003
Publication Date:
02 March 2004 (online)
Abstract
We have evaluated the effect of 3,4-secoisopimar-4(18),7,15-triene-3-oic acid (compound 1), isolated from the aerial parts of Salvia cinnabarina, on the contractile response elicited by electrical field stimulation (EFS) in the rat isolated urinary bladder. Compound 1 (10 - 7 - 10 - 4 M) produced a concentration-dependent inhibition of the EFS contractile response without modifying the contractions produced by exogenous acetylcholine (10 - 6 M). A number of antagonists/inhibitors including a combination of atropine (10 - 6 M), phentolamine (10 - 6 M), propranolol (10 - 6 M) and hexamethonium (10 - 4 M), the NK1 receptor antagonist SR140333 (10 - 7 M) plus the NK2 receptor antagonist SR48968 (10 - 6 M), naloxone (10 - 6 M), verapamil (10 - 7 M), capsazepine (10 - 5 M) and the CB1 receptor antagonist SR141716A (10 - 6 M) did not modify the inhibitory effect of compound 1. However, the nitric oxide (NO) synthase inhibitor L-NAME (3 × 10 - 4 M), significantly reduced the inhibitory effect of compound 1. It is concluded that compound 1 inhibits rat bladder contractility with a mechanism involving, at least in part, NO production.
References
- 1 Chadefaud M, Emberger L. Traite de Botanique (Sistematique). Masson et Compagnie Editeurs Paris; 1960: 839
- 2 Dobrynin V N, Kolosov M N, Chernov B K, Derbentseva N A. Antimicrobial substances of Salvia officinalis . Kmim Prip Soedin. 1976; 5 686-7
- 3 Hitokoto H, Morozumi S, Wauke T, Sakai S, Kurata H. Inhibitory effects of spices on growth and toxin production of toxigenic fungi. Appl Environ Microbiol. 1980; 39 818-22
- 4 Topcu G, Tan N, Kokdil G, Ulubelen A. Terpenoids from Salvia glutinosa . Phytochemistry. 1997; 45 1293-4
-
5 Rodriguez-Hahn L, Esquivel B, Cardnas J. Clerodane diterpenes in Labiatae. In: Zechmeister L, editor
Progress in the Chemistry of Organic Natural Products . Vol LXIII Heidelberg; Springer 1994: 162 - 6 Ulubelen A, Evren N, Tuzlaci E, Johansson C. Diterpenoids from the roots of Salvia hypargeia . J Nat Prod. 1988; 51 1178-83
-
7 Rodriguez-Hahn L, Esquival B, Cardenas J, Ramamoorthy T S. In: Harley RM, Reynolds T, editors
Advances in Labiatae Science . Royal Botanic Gardens Kew Richmond; 1992: 335-347 - 8 Rustaiyan A, Koussari S. Further sesterterpenes from Salvia hypoleuca . Phytochemistry. 1988; 27 1767-9
- 9 Gali-Muhtasib H, Hilan C, Khater C. Traditional uses of Salvia libanotica (East Mediterranean sage) and the effects of its essential oils. J Ethnopharmacol. 2000; 7 513-20
- 10 Lu Y, Foo L Y. Polyphenolics of Salvia. A review. Phytochemistry. 2002; 59 117-40
-
11 Epling C. A Revision of Salvia, subgenus Calosphace. In: Fedde F, editor
Repertorium Specierum Novarum Regni Vegetabilis . Vol. CX Berlin; Dahlem 1939: 139 - 12 Bisio A, Ciarallo G, Romussi G, Fontana N, Mascolo N, Capasso R, Biscardi D. Chemical composition of essential oils from some Salvia species. Phytother Res. 1998; 12 S117-20
- 13 Romussi G, Ciarallo G, Bisio A, Fontana N, De Simone F, De Tommasi N, Mascolo N, Pinto L. A new diterpenoid with antispasmodic activity from Salvia cinnabarina . Planta Med. 2001; 67 153-5
- 14 Capasso R, Tavares I A. Effect of the flavonoid galangin on urinary bladder rat contractility in-vitro . J Pharm Pharmacol. 2002; 54 1147-50
- 15 Rigoni M, Trevisani M, Gazzieri D, Nadaletto R, Tognetto M, Creminon C, Davis J B, Campi B, Amadesi S, Geppetti P, Harrison S. Neurogenic responses mediated by vanilloid receptor-1 (TRPV1) are blocked by the high affinity antagonist, iodo-resiniferatoxin. Br J Pharmacol. 2003; 138 977-85
- 16 Uruno T, Shirane M, Wada K, Tsunematsu R, Nagahamaya K, Matsuoka Y, Sunagane N, Kubota K. Possible mechanisms of action of the antispasmodic agent tiropramide in the isolated detrusor from rats. Jpn J Pharmacol. 1992; 60 75-80
- 17 Moncada S, Palmer R M, Higgs E A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991; 43 109-42
- 18 Elliot R A, Norman R I, Parker S G, Whitaker R P, Castleden C M. Effect of treatment with calcium antagonists in vitro and in vivo on the contractile response of isolated rat and human detrusor muscle. Clin Sci. 1996; 91 467-74
- 19 Izzo A A, Mascolo N, Borrelli F, Capasso F. Excitatory transmission to the circular muscle of the guinea-pig ileum: evidence for the involvement of cannabinoid CB1 receptors. Br J Pharmacol. 1998; 124 363-8
- 20 Nocerino E, Izzo A A, Borrelli F, Capasso F, Capasso R, Pinto A, Sautebin L, Mascolo N. Relaxant effect of capsazepine in the isolated rat ileum. Naunyn Schmiedebergs Arch Pharmacol. 2002; 365 187-92
Prof. Nicola Mascolo
Department of Experimental Pharmacology
University of Naples ”Federico II”
via D. Montesano 49
80131 Naples
Italy
Phone: +39-081-678465
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Email: nmascolo@unina.it