Planta Med 2004; 70(2): 189-192
DOI: 10.1055/s-2004-815502
Letter
© Georg Thieme Verlag Stuttgart · New York

Species Identification from Ginseng Drugs by Multiplex Amplification Refractory Mutation System (MARMS)

Shu Zhu1, 2 , Hirotoshi Fushimi1 , Shaoqing Cai2 , Katsuko Komatsu1, 3
  • 1Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan
  • 2Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, P.R. China
  • 321st Century COE Program, Toyama Medical and Pharmaceutical University, Toyama, Japan
This work was supported by a Grant-in-Aid for Scientific Research (B), No. 11695086 in 1999 - 2001 and No. 14406030 in 2002 - 2003 from Japan Society for the Promotion of Science, and for the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan
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Publikationsverlauf

Received: July 16, 2003

Accepted: October 11, 2003

Publikationsdatum:
02. März 2004 (online)

Abstract

The multiplex amplification refractory mutation system (MARMS) was applied to the identification of 5 Panax species (P. ginseng, P. japonicus, P. quinquefolius, P. notoginseng and P. vietnamensis). A set of specific primers, including 2-pair primers on chloroplast trnK gene and nuclear 18S rRNA gene regions, respectively, was designed and synthesized for each species on the basis of species-specific sequences of the 2 genes. By using 5 sets of specific primers, in turn, PCR amplifications were performed with total DNA extracted from 5 Panax species as template under appropriate condition, and each resulting product was detected by agarose gel electrophoresis. The results showed that two expected fragments, one from trnK gene and another from 18S rRNA gene regions, were observed simultaneously only when the set of species-specific primers encountered template DNA of the corresponding species. This assay could give more reliable results for identification of not only 5 Panax species but also corresponding Ginseng drugs by simultaneous detection of 4-site nucleotide differences on 2 completely different genes.

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Dr. Katsuko Komatsu

Institute of Natural Medicine

Toyama Medical and Pharmaceutical University

2630 Sugitani

Toyama

930-0194 Toyama

Japan

Telefon: +81-76-434-7645

Fax: +81-76-434-5064

eMail: katsukok@ms.toyama-mpu.ac.jp