Involvement of the Plasma Membrane Ca2+-ATPase in the Short-Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

G. Romani; M. C. Bonza; I. Filippini; M. Cerana; N. Beffagna; M. I. De Michelis

doi:10.1055/s-2004-817848

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2004; 6(2): 192-200
DOI: 10.1055/s-2004-817848

Original Paper

Georg Thieme Verlag Stuttgart · New York

Involvement of the Plasma Membrane Ca²⁺-ATPase in the Short-Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

G. Romani¹ , M. C. Bonza¹ , I. Filippini¹ , M. Cerana¹ , N. Beffagna¹ , M. I. De Michelis¹

¹Istituto di Biofisica del CNR-Sezione di Milano, Dipartimento di Biologia, Università degli Studi di Milano, Milano, Italy

Abstract

Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment. The analysis of OG effects on Ca²⁺ fluxes shows that OG influence both Ca²⁺ influx and Ca²⁺ efflux (measured as ⁴⁵Ca²⁺ fluxes) in a complex way. During the first 10 - 15 min, OG stimulate Ca²⁺ influx and decrease its efflux, while at successive times of treatment, OG cause an increase of Ca²⁺ efflux and a slight decrease of its influx. Treatment with sub-µM concentrations of eosin yellow (EY), which selectively inhibits the Ca²⁺-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca²⁺ efflux. EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high. The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca²⁺-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM. During the same time span, OG do not influence the expression of At-ACA8, the main isoform of PM Ca²⁺-ATPase in suspension-cultured A. thaliana cells, and of CaM genes. Overall, the reported results demonstrate that the PM Ca²⁺-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst.

Key words

Arabidopsis thaliana cells - Ca²⁺ fluxes - calmodulin - plasma membrane Ca²⁺-ATPase - oligogalacturonides - oxidative burst

Full Text

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G. Romani

Istituto di Biofisica del CNR-Sezione di Milano
Dipartimento di Biologia
Università degli Studi di Milano

Via Celoria, 26

20133 Milano

Italy

Email: giulia.romani@unimi.it

Section Editor: G. Thiel