Transkriptionelle Aktivität der Surfactant-Apoproteine A1 und A2 in nicht kleinzelligen Bronchialkarzinomen und tumorfreien Lungengeweben

Zusammenfassung

In dieser Arbeit wurde das transkriptionelle Verhältnis der Surfactant-Apoproteine A1 und A2 (SP-A1 & SP-A2) im Tumor und in tumorfreien Lungengeweben von Patienten mit nicht kleinzelligen Bronchialkarzinomen untersucht. Material und Methoden: Unfixierte schockgefrorene Proben aus Lungenresektionspräparaten von 21 Patienten mit Adenokarzinomen der Lunge und 6 Patienten mit Plattenepithelkarzinomen (Kontrollgruppe) wurden untersucht. Hierzu wurde RT-PCR unter Nutzung eines SP-A1/SP-A2-Konsensusfragmentes gefolgt von enzymatischen Restriktionsanalysen durchgeführt. Ergebnisse: Tumorhaltige Gewebe der Adenokarzinome zeigten höhere SP-A1/SP-A2-Verhältnisse als tumorfreie Gewebe. Die Unterschiede der Expression von SP-A1/SP-A2 in den intraindividuellen tumorhaltigen und tumorfreien Geweben der untersuchten Adenokarzinome ist statistisch signifikant (p = 0,05). Plattenepithelkarzinome zeigten dieses nicht. Schlussfolgerung: Eine neue Methode zur Untersuchung der transkriptionellen Aktivität der Surfactant-Apoproteine A1 und A2 in schockgefrorenen Lungengeweben wurde etabliert. Es wurde gezeigt, dass Adenokarzinomgewebe höhere SP-A1/SP-A2-Verhältnisse aufweisen als entsprechende tumorfreie Gewebe und dass die Variationsbreite der SP-A-mRNA-Expression in den Fällen höheren Tumorgradings zunimmt.

Abstract

Objective of this study was to investigate the balance of surfactant-apoprotein A1 and A2 (SP-A1 & SP-A2) at transcriptional level in tumor and tumor-free lung-tissues of patients with non-small-cell lung-carcinomas. Materials and Methods: Nonfixed snap-frozen tumor and tumor-free sections of lungs, excised from 21 patients with adenocarcinomas of the lung and 6 patients with squamous cell carcinomas (control group) were analyzed. This was accomplished by RT-PCR using a SP-A1/SP-A2 consensus sequence, followed by enzymatic restriction. Results: Tumor-containing tissues of adenocarcinomas showed higher SP-A1/SP-A2 ratios than the tumor free tissues. There was a significant difference in expression of SP-A1/SP-A2-mRNA in intra-individual tumor and tumor-free lung-tissues of adenocarcinoma patients (p = 0,05). In cases of squamous cell carcinomas this was not observed. Conclusions: A novel method to investigate the transcriptional activity of surfactant-apoproteins A1 and A2 in snap-frozen lung tissues was established. It was shown, that adenocarcinoma-tissues display higher SP-A1/SP-A2 than the corresponding tumor-free tissues and that the variation of SP-A-mRNA expression rises in cases of higher tumor-grading.

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